Long-term phenotypic correction of rodent hemiparkinsonism by gene therapy using genetically modified myoblasts
| Autor: | Xiao Liu, Dan Xu, Sheng-Di Chen, Zhongyi Jiang, Zhen-Guo Liu, Y C Zhao, Liang Cao, Zhongcheng Zheng |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Tyrosine 3-Monooxygenase Dopamine Genetic enhancement Transgene Striatum Biology Internal medicine Gene expression Genetics medicine Animals Myocyte Transgenes Molecular Biology Cells Cultured Tyrosine hydroxylase Muscles Gene Transfer Techniques Parkinson Disease Genetic Therapy Immunohistochemistry Rats Genetically modified organism Phenotype Endocrinology Molecular Medicine medicine.drug |
| Zdroj: | Gene Therapy. 7:445-449 |
| ISSN: | 1476-5462 0969-7128 |
| Popis: | Rat myoblasts were genetically modified to express tyrosine hydroxylase (TH) and produce dopamine in culture. Implanting TH gene-transfected myoblasts into the denervated striatum of 6-OHDA-lesioned rats significantly decreased rotational asymmetry by 50 to approximately 60%. Improvement persisted for up to 13 months. Genetically modified cells could survive and express transgene in the striatum as demonstrated by RT-PCR and immunohistochemical stain-ing. The dopamine content in the striatum tissue of the gene therapy group recovered to 49% of the normal level and was 25-fold higher than that of a control group receiving parental cells. Neither tumor formation nor immunorejection was observed in this study. These results show that myoblasts may be useful as gene carriers for ex vivo gene therapy in the CNS. Gene Therapy (2000) 7, 445-449. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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