Proteomics of Human Aqueous Humor

Autor: Raja Sekhar Nirujogi, Renu Goel, Pavithra Rajagopalan, Sneha M. Pinto, Mahashweta Dash, Bipin G. Nair, Abhijith K. Anil, Yashwanth Subbannayya, Subramanian Shankar, Krishna R Murthy, Gajanan Sathe, Harsha Gowda, B. L. Somani, Aditi Chatterjee, Gourav Dey, Dhanashree S. Kelkar, Akhilesh Pandey, Lavanya Balakrishnan, Shukti Chakravarti, Jayshree Advani, Nandini A. Sahasrabuddhe, T. S. Keshava Prasad, Praveen R Murthy, Srikanth S. Manda
Rok vydání: 2015
Předmět:
Zdroj: OMICS: A Journal of Integrative Biology. 19:283-293
ISSN: 1557-8100
1536-2310
Popis: The aqueous humor is a colorless, transparent fluid that fills the anterior chamber of the eye. It plays an important role in maintaining the intraocular pressure and providing nourishment to the lens and cornea. The constitution of the aqueous humor is controlled by the blood-aqueous barrier. Though this ocular fluid has been extensively studied, its role in ocular physiology is still not completely understood. In this study, aqueous humor samples were collected from 250 patients undergoing cataract surgery, subjected to multiple fractionation strategies and analyzed on a Fourier transform LTQ-Orbitrap Velos mass spectrometer. In all, we identified 763 proteins, of which 386 have been identified for the first time in this study. Sorbitol dehydrogenase (SORD), filensin (BFSP1), and phakinin (BFSP2) are some of the proteins that have not been previously reported in the aqueous humor. Gene Ontology analysis revealed 35% of the identified proteins to be extracellular, with a majority of them involved in cell communication and signal transduction. This study comprehensively reports 386 novel proteins that have important potential as biomarker candidates for future research into personalized medicine and diagnostics aimed towards improving visual health.
Databáze: OpenAIRE
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