Sperm-borne small RNA regulate α-tubulin acetylation and epigenetic modification of early bovine somatic cell nuclear transfer embryos
| Autor: | Mengyun Wang, Zhenzi Zuo, Zhengqing Liu, Fang Qiao, Xiaonan Ma, Zhihan Niu, Yong Zhang, Yue Du, Suzhu Qing, Pengxiang Qu, Yongsheng Wang, Ying Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Nuclear Transfer Techniques Embryology Small RNA sperm-borne small RNA Somatic cell Fluorescent Antibody Technique Apoptosis Biology Polymerase Chain Reaction cloning efficiency Epigenesis Genetic H3K9me3 Histones 03 medical and health sciences Histone H3 0302 clinical medicine Tubulin Genetics Animals Epigenetics Molecular Biology Cloning the first cleavage Microscopy Confocal 030219 obstetrics & reproductive medicine urogenital system Embryogenesis Obstetrics and Gynecology Acetylation Embryo Cell Biology Spermatozoa α-tubulin K40 acetylation Cell biology Blastocyst 030104 developmental biology Reproductive Medicine embryonic development embryonic structures Somatic cell nuclear transfer Cattle Protein Processing Post-Translational Developmental Biology |
| Zdroj: | Qu, P, Zuo, Z, Liu, Z, Niu, Z, Zhang, Y, Du, Y, Ma, X, Qiao, F, Wang, M, Zhang, Y, Qing, S & Wang, Y 2019, ' Sperm-borne small RNA regulate α-tubulin acetylation and epigenetic modification of early bovine somatic cell nuclear transfer embryos ', Molecular Human Reproduction . https://doi.org/10.1093/molehr/gaz023 |
| Popis: | Accumulated evidence indicates that sperm-borne small RNA plays a crucial role in embryonic development, especially the absence of the sperm-borne small RNA might be a major cause of the abnormal development of cloned embryos. In this study, we found that sperm-borne small RNA can affect abnormal pronuclear-like structures, postpone the timing of first embryo cleavage and enhance developmental competence of bovine somatic cell nuclear transfer (SCNT) embryos. In addition, the supplementation of sperm-borne small RNA can significantly increase live birth rates and decrease the birth weights of cloned offspring. To investigate the underlying mechanisms, the levels of α-tubulin K40 acetylation (Ac α-tubulin K40) and histone H3 lysine 9 trimethylation (H3K9me3) during early embryo development were investigated in SCNT embryos with sperm-borne small RNA supplementation (termed as T-NT), compared to those normal SCNT embryos and embryos obtained from standard IVF. The results showed that sperm-borne small RNA can significantly decrease the H3K9me3 levels at the pronuclear and two-cell stages, while significantly increase Ac α-tubulin K40 levels at anaphase and telophase of bovine SCNT embryos during the first cleavage. Collectively, our study for the first time demonstrates that sperm-borne small RNA plays a crucial role in the developmental competence of SCNT embryos by regulating H3K9me3 and Ac α-tubulin K40. Further studies will be required to determine how sperm small RNA regulate the H3K9me3 and Acα-tubulin K40. Our study suggests that the supplementation of sperm-borne small RNA is a potential application to improve the cloning efficiency. |
| Databáze: | OpenAIRE |
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