Expression and characterization of recombinant Japanese encephalitis virus NS1 protein in Drosophila S2 cell
| Autor: | Yize Li, Félix A. Rey, Nelly Kieffer, Dorian Counor, Vincent Deubel, Marie Flamand |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
viruses
lcsh:Medicine General Biochemistry Genetics and Molecular Biology Virus law.invention Antigen law medicine lcsh:Science chemistry.chemical_classification biology business.industry Schneider 2 cells lcsh:R virus diseases General Medicine biochemical phenomena metabolism and nutrition Japanese encephalitis medicine.disease biology.organism_classification Virology Flavivirus chemistry Recombinant DNA lcsh:Q business Glycoprotein Encephalitis |
| Zdroj: | BMC Proceedings, Vol 2, Iss Suppl 1, p P36 (2008) |
| ISSN: | 1753-6561 |
| DOI: | 10.1186/1753-6561-2-s1-p36 |
| Popis: | Background Japanese encephalitis virus (JEV) is a member of the genus Flavivirus, family Flaviviridae. Flavivirus NS1 glycoproteins are essential proteins, which exhibit a high degree of sequence homology. The NS1 protein is secreted from infected cells as a soluble hexamer and can induce protective immune response in mice against lethal encephalitis. However, its role in neuroinvasion of JEV remains unknown. Our aim is to identify the role of NS1 protein in JEV neuropathogenesis. In this study, we have produced recombinant JEV NS1 protein in Drosophila S2 cells and have characterized its biochemical, biological and antigenic properties. |
| Databáze: | OpenAIRE |
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