Protozoan Parasites and Leafy Greens in Marrakech: Study of Occurrence Using a Molecular Method
| Autor: | Eva Nast, Jamaleddine Hafid, Laila Hoummadi, Dominique Aubert, Atika Madline, Isabelle Villena, Salma Berrouch, Stéphanie La Carbona, Sandie Escotte-Binet |
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| Přispěvatelé: | Faculté des Sciences et Techniques Marrakech, Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle (ESCAPE), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA), Centre Hospitalier Universitaire de Reims (CHU Reims), ACTALIA [Villers-Bocage] |
| Rok vydání: | 2021 |
| Předmět: |
Giardiasis
Veterinary medicine Cryptosporidiosis Cryptosporidium 03 medical and health sciences [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases parasitic diseases Animals Humans Parasites Leafy ComputingMilieux_MISCELLANEOUS 030304 developmental biology [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health 0303 health sciences biology 030306 microbiology Protozoan diseases Oocysts Toxoplasma gondii DNA Protozoan Contamination biology.organism_classification 3. Good health [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Giardia duodenalis Parasitology Toxoplasma Contaminated food |
| Zdroj: | Acta Parasitologica Acta Parasitologica, Springer Verlag, 2021, ⟨10.1007/s11686-021-00488-z⟩ |
| ISSN: | 1896-1851 1230-2821 |
| DOI: | 10.1007/s11686-021-00488-z |
| Popis: | Purpose The aim of this study was to assess the presence of T. gondii, Cryptosporidium spp. oocysts, and G. duodenalis cysts, in three leafy greens (coriander, lettuce, and parsley) commonly consumed raw. Despite the recognition of the association between the parasitic illnesses and the consumption of contaminated food, there is still a lack of studies investigating the occurrence of parasitic contamination in food matrices. Methods A total of 152 leafy green samples were collected in Marrakech from April 2018 to October 2019. Parasites were eluted and concentrated before detection of their DNA by real-time qPCR. Results The analysis revealed an overall rate of contamination of 32.2% (49/152), with 29.6% (45/152) positive for T. gondii and 2.6% (4/152) for G. duodenalis, while none was positive for Cryptosporidium spp. Conclusion The results showed that humans can be exposed to protozoan parasites through vegetables consumption. Further investigations can be performed to acquire new epidemiological data to assess the public health impact of these protozoan diseases in Morocco. |
| Databáze: | OpenAIRE |
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