Biomimetic Microgels with Controllable Deformability Improve Healing Outcomes
| Autor: | Erin P. Sproul, Colleen Roosa, Seema Nandi, Ashley C. Brown, Luisa Schreck |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Platelet Morphology biology Chemistry digestive oral and skin physiology technology industry and agriculture Biomedical Engineering macromolecular substances 02 engineering and technology Clot retraction 021001 nanoscience & nanotechnology Article General Biochemistry Genetics and Molecular Biology Fibrin Biomaterials 03 medical and health sciences 030104 developmental biology Tissue ischemia In vivo Hemostasis biology.protein Biophysics Platelet 0210 nano-technology Wound healing |
| Zdroj: | Adv Biosyst |
| ISSN: | 2366-7478 |
| Popis: | Platelets mediate hemostasis by aggregating and binding to fibrin to promote clotting. Over time, platelets contract the fibrin network to induce clot retraction, which contributes to wound healing outcomes by increasing clot stability and improving blood flow to ischemic tissue. In this study, we describe the development of hollow platelet-like particles (PLPs) that mimic the native platelet function of clot retraction in a controlled manner and demonstrate that clot retraction-inducing PLPs promote healing in vivo. PLPs are created by coupling fibrin-binding antibodies to CoreShell (CS) or hollow N-isopropylacrylamide (NIPAm) microgels with varying degrees of shell crosslinking. We demonstrate that hollow microgels with loosely crosslinked shells display a high degree of deformability and mimic activated platelet morphology, while intact CS microgels and hollow microgels with increased crosslinking in the shell do not. When coupled to a fibrin-binding antibody to create PLPs, hollow particles with low degrees of shell crosslinking cause fibrin clot collapse in vitro, recapitulating the clot retraction function of platelets, while other particle types do not. Furthermore, hollow PLPs with low degrees of shell crosslinking improve some wound healing outcomes in vivo. |
| Databáze: | OpenAIRE |
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