Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium
| Autor: | Hend Altaib, Yuka Ozaki, Tomoya Kozakai, Kouta Sakaguchi, Izumi Nomura, Tohru Suzuki |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Microbiology Resource Announcements |
| ISSN: | 2576-098X |
| Popis: | A series of Bifidobacterium - Escherichia coli shuttle vectors (pKO403- lacZ′ -Cm, pKO403- lacZ′ -Sp, pKO403- lacZ′ -p15A) were constructed based on the pKO403 backbone, which carries a temperature-sensitive replication origin. These vectors carry the lacZ′ α fragment, overhung by two facing type IIS restriction sites, for blue-white selection and seamless gene cloning. |
| Databáze: | OpenAIRE |
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