Humoral immune response in Oreochromis niloticus: modified direct agglutination test

Autor: Paulo Fernandes Marcusso, Flávio Ruas de Moraes, Flávia Campos Freitas Vieira, Marina Tie Shimada, Elaine C. Souza, Gustavo da Silva Claudiano, Silas Fernandes Eto, Dayanne Carla Fernandes, Julieta Rodini Engrácia de Moraes, Thiago Fernandes Alves Silva, Rogério Salvador
Přispěvatelé: Universidade Estadual Paulista (Unesp)
Rok vydání: 2014
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Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1618-565X
1618-5641
Popis: Made available in DSpace on 2018-12-11T16:39:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-05-01 Understanding the functional mechanisms of the humoral and cellular immune responses among teleost broadens the possibilities for intervention and improvement of vaccination methods. The aim of this study was to evaluate the dynamics of the humoral immune response, hematological biochemical changes, and electrophoretic profile of proteins in Oreochromis niloticus, when submitted to antigen stimulation using sheep erythrocytes in a single dose and with booster doses. Seventy O. niloticus were distributed in two groups (n = 35): one received a single dose and the other received two doses of antigen. Evaluations were made at the following predetermined times: zero (pre-immune, i.e., before inoculation with the first injection of sheep erythrocytes, negative control) and on 7th, 14th, 21st, and 28th days after the first immunization to evaluate the hemogram, biochemistry, and electrophoresis of proteins and direct hemagglutination. Twenty-eight days after antigen inoculation, it was observed that there had been an increase in the serum antibody titer and fractions gamma globulins after second antigen stimulation. The results suggest that teleost fish have the capacity to produce antibodies from a single stimulation, and a second antibody production wave after second stimulation. Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), Via Prof. Paulo Donato Castellane, km 05 Department of Technology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), Via Prof. Paulo Donato Castellane, km 05 Department of Veterinary Pathology, State University of the North of Paraná (Uenp), Luiz Meneghel Campus, Rodovia BR-369 km Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), Via Prof. Paulo Donato Castellane, km 05 Department of Technology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), Via Prof. Paulo Donato Castellane, km 05
Databáze: OpenAIRE
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