Chondrogenic differentiation of ChM‐I gene transfected rat bone marrow‐derived mesenchymal stem cells on 3‐dimensional poly ( L ‐lactic acid) scaffold for cartilage engineering

Autor: Yang Liu, Zhiqiang Wang, Zhiyong Wei, Shuangchun Xing, Yu-qiang Hu, Ya Feng, Lijie Zhai, Xinhui Wang, Chen Jiang, Min Qi, Wei Sun, Jing Liu
Rok vydání: 2014
Předmět:
Zdroj: Cell Biology International. 39:300-309
ISSN: 1095-8355
1065-6995
DOI: 10.1002/cbin.10393
Popis: We have explored the role of Chondromodulin-I (ChM-I) in chondrogenesis of bone marrow-derived mesenchymal stem cells (BMSCs) in 3-dimensional (3D) scaffold for cartilage tissue engineering. BMSCs of Sprague Dawley (SD) rats were cultured on poly-(L-lactic acid) [PLLA] scaffolds with different pore sizes (80-200 μm, 200-450 μm) with or without surface modification by chitosan. Cell viability, proliferation, and morphology were measured using confocal microscope and the CCK-8 method. Untransfected BMSCs, BMSCs expressing pcDNA3.1(+), BMSCs expressing plasmid pcDNA3.1 (+)/ChM-I were cultured on 3D scaffolds in standard growth medium or transforming growth factor-β1 (TGF-β1) supplemented chondrogenic induction medium in vitro for 3 weeks and the expression of collagen type II was determined. Cell-scaffolds constructs were implanted subcutaneously for 3 months in vivo. BMSCs had a higher viability and proliferation in PLLA scaffolds of pore size 200-450 μm than that of 80-200 μm, and surface modification with chitosan did not enhance cell attachment. The ChM-I gene enhanced chondrogenesis and increased collagen type II synthesis. Immunohistochemistry from in vivo study showed enhanced cartilage regeneration in BMSCs expressing pcDNA3.1 (+)/ChM-I on 3D PLLA scaffolds. It also demonstrated that TGF-β1 might promote chondrogenesis of rat BMSCs by synergizing with the ChM-I gene. ChM-I could be beneficial to future applications in cartilage repair.
Databáze: OpenAIRE
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