Endothelin inhibits the atrial natriuretic factor stimulated cGMP production by activating the protein kinase C in rat aortic smooth muscle cells
| Autor: | Rama K. Jaiswal |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Biophysics Aorta Thoracic Peptide hormone Biology Biochemistry Muscle Smooth Vascular Piperazines chemistry.chemical_compound Cytosol Atrial natriuretic peptide Internal medicine 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine medicine Animals Molecular Biology Cyclic GMP Protein Kinase Inhibitors Protein kinase C Cells Cultured Protein Kinase C Kinase Endothelins Rats Inbred Strains Cell Biology Isoquinolines Endothelin 1 Rats Enzyme Activation Kinetics Endocrinology chemistry cardiovascular system Phorbol Tetradecanoylphorbol Acetate Endothelin receptor Protein Kinases Atrial Natriuretic Factor |
| Zdroj: | Biochemical and biophysical research communications. 182(1) |
| ISSN: | 0006-291X |
| Popis: | Summary Preincubation of rat thoracic aortic smooth muscle cells with endothelin inhibits the atrial natriuretic factor (ANF)-induced cGMP accumulation in these cells in a concentration dependent manner. The maximal inhibition of 64% was afforded by 1×10 −6 M endothelin and the half maximal inhibition (IC 50 ) was achieved with 1×10 −9 M endothelin. Endothelin (1×10 −6 M) also increased the plasma membrane bound protein kinase C (PKC) activity by 4 fold. Hormone-dependent increase in PKC activity was limited to plasma membranes only and some decrease in cytosolic PKC activity was observed. However, phorbol 12-myristate 13-acetate (PMA) (1×10 −6 M) provoked a total loss of cytosolic PKC activity and a net gain in membranous PKC activity indicative of the translocation of the enzyme. Pretreatment of these cells with H-7, a PKC inhibitor, released the endothelin and PMA-mediated attenuation of ANF-stimulated cGMP formation. These results suggest that PKC is involved in the regulation of ANF-induced cGMP accumulation and that the vasoconstrictor activity of endothelin might involve inhibition of the vasorelaxant activity of ANF through the inhibition of cGMP accumulation in smooth muscle cells (SMCs) of the rat aorta. |
| Databáze: | OpenAIRE |
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