Increasing the miR-126 expression in the peripheral blood of patients with diabetic foot ulcers treated with maggot debridement therapy
| Autor: | Xinjuan Sun, Jie Zhang, Ai-ping Wang, Dong Mei Gu, Jin’an Chen, Zhi Wei Hu, Lei Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Complementary Therapies
Male 0301 basic medicine Bodily Secretions China medicine.medical_specialty Pathology Angiogenesis Endocrinology Diabetes and Metabolism medicine.medical_treatment Population Gastroenterology 03 medical and health sciences Hospitals Urban Endocrinology Internal medicine Diabetes mellitus Human Umbilical Vein Endothelial Cells Internal Medicine medicine Animals Germ-Free Life Humans education Cells Cultured Aged education.field_of_study Debridement business.industry Maggot Diptera Type 2 Diabetes Mellitus Middle Aged medicine.disease Diabetic foot Coculture Techniques Diabetic Foot Up-Regulation Biological Therapy MicroRNAs 030104 developmental biology Diabetic foot ulcer Diabetes Mellitus Type 2 Gene Expression Regulation Larva Female Endothelium Vascular business |
| Zdroj: | Journal of Diabetes and its Complications. 31:241-244 |
| ISSN: | 1056-8727 |
| DOI: | 10.1016/j.jdiacomp.2016.07.026 |
| Popis: | miR-126 may increase angiogenesis in patients with diabetic foot ulcers (DFUs) treated with maggot debridement therapy (MDT).Real-time quantitative PCR was used to detect expression of miR-126 mRNA in the peripheral blood among the non-diabetic population, type 2 diabetes mellitus patients without DFU, and patients with DFUs of type 2 diabetes mellitus. The expression of miR-126 mRNA in the peripheral blood of patients with DFUs was observed before and after MDT. Finally, human umbilical vein endothelial cells (HUVEC) were utilized to explore miR-126 mRNA expression with maggot excretions/secretions (ES).In the patients with DFUs, the miR-126 mRNA expression level in the peripheral blood was less than that type 2 diabetes mellitus patients without DFU, and much lower than that in the non-diabetic population (P0.001). The miR-126 expression level was significantly increased in those DFU patients treated with MDT (P0.05). Finally, using HUVEC co-cultured with ES, we showed the ES increased miR-126 expression in vitro (P0.001).MDT upregulates the miR-126 expression in the peripheral blood of patients with DFUs. |
| Databáze: | OpenAIRE |
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