S antigen specific effector T cell activation detected by cytokine flow cytometry
| Autor: | J P, Morgan, R A, Robins, H S, Dua, P J, Tighe |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
T-Lymphocytes
medicine.medical_treatment T cell Pilot Projects Biology Lymphocyte Activation Peripheral blood mononuclear cell Autoimmune Diseases Flow cytometry Uveitis Interferon-gamma Cellular and Molecular Neuroscience CD28 Antigens medicine Humans Interferon gamma Arrestin medicine.diagnostic_test Effector CD69 T lymphocyte Flow Cytometry Sensory Systems Ophthalmology Cytokine medicine.anatomical_structure Immunology Scientific Correspondence medicine.drug |
| Zdroj: | British Journal of Ophthalmology. 86:517-520 |
| ISSN: | 0007-1161 |
| DOI: | 10.1136/bjo.86.5.517 |
| Popis: | Background/aims: Effector T cell activation is particularly important in the initiation of autoimmune uveitis. This pilot study seeks to demonstrate activation of human peripheral effector T cells in response to the uveitis candidate autoantigen, retinal S antigen (SAg), using cytokine flow cytometry (CFC). Methods: Peripheral blood mononuclear cell (PBMC) suspensions from uveitis patients and controls were stimulated with bovine SAg. Activation responses were detected by CFC. Results: Electronic gating enabled analysis of CD69+, IFN-γ+ CD4+ lymphocytes. An SAg specific response was detectable in four of 13 patients and four of eight controls. Conclusion: SAg specific, peripheral, effector T cell activation can be detected by CFC. Similar levels of responsiveness were seen in patient and control groups. More detailed cytokine profiling may demonstrate functional differences between the groups. |
| Databáze: | OpenAIRE |
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