Mechanisms of direct inhibition of the respiratory sulfate-reduction pathway by (per)chlorate and nitrate
| Autor: | Hans K. Carlson, Adam M. Deutschbauer, Anna Engelbrektson, Mark R. Mullan, Amrita B. Hazra, Adam P. Arkin, Nicholas B. Justice, Magdalena K. Stoeva, Jennifer V. Kuehl, John D. Coates, Morgan N. Price, Anthony T. Iavarone, Andrew Sczesnak |
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| Rok vydání: | 2014 |
| Předmět: |
Proteomics
Desulfovibrio alaskensis 16S Technology Mutant Sulfides Biology Polymerase Chain Reaction Microbiology Perchlorate chemistry.chemical_compound Nitrate RNA Ribosomal 16S Ecosystem Ecology Evolution Behavior and Systematics Derepression Ribosomal Nitrates Perchlorates Sulfur-Reducing Bacteria Sulfates Gene Expression Profiling Chlorate Genomics Biological Sciences biology.organism_classification Regulon Gene Expression Regulation chemistry Biochemistry Mutation Chlorates DNA Transposable Elements RNA Original Article Desulfovibrio NAD+ kinase Oxidation-Reduction Environmental Sciences |
| Zdroj: | Carlson, HK; Kuehl, JV; Hazra, AB; Justice, NB; Stoeva, MK; Sczesnak, A; et al.(2015). Mechanisms of direct inhibition of the respiratory sulfate-reduction pathway by (per)chlorate and nitrate. ISME Journal, 9(6), 1295-1305. doi: 10.1038/ismej.2014.216. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/22k3339m The ISME journal, vol 9, iss 6 |
| ISSN: | 1751-7370 1751-7362 |
| DOI: | 10.1038/ismej.2014.216 |
| Popis: | © 2015 International Society for Microbial Ecology. All rights reserved. We investigated perchlorate (ClO 4 -) and chlorate (ClO 3 -) (collectively (per)chlorate) in comparison with nitrate as potential inhibitors of sulfide (H 2 S) production by mesophilic sulfate-reducing microorganisms (SRMs). We demonstrate the specificity and potency of (per)chlorate as direct SRM inhibitors in both pure cultures and undefined sulfidogenic communities. We demonstrate that (per)chlorate and nitrate are antagonistic inhibitors and resistance is cross-inducible implying that these compounds share at least one common mechanism of resistance. Using tagged-transposon pools we identified genes responsible for sensitivity and resistance in Desulfovibrio alaskensis G20. We found that mutants in Dde-2702 (Rex), a repressor of the central sulfate-reduction pathway were resistant to both (per)chlorate and nitrate. In general, Rex derepresses its regulon in response to increasing intracellular NADH:NAD + ratios. In cells in which respiratory sulfate reduction is inhibited, NADH:NAD + ratios should increase leading to derepression of the sulfate-reduction pathway. In support of this, in (per)chlorate or nitrate-stressed wild-type G20 we observed higher NADH:NAD + ratios, increased transcripts and increased peptide counts for genes in the core Rex regulon. We conclude that one mode of (per)chlorate and nitrate toxicity is as direct inhibitors of the central sulfate-reduction pathway. Our results demonstrate that (per)chlorate are more potent inhibitors than nitrate in both pure cultures and communities, implying that they represent an attractive alternative for controlling sulfidogenesis in industrial ecosystems. Of these, perchlorate offers better application logistics because of its inhibitory potency, solubility, relative chemical stability, low affinity for mineral cations and high mobility in environmental systems. |
| Databáze: | OpenAIRE |
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