Detection and differentiation of antibodies to human T-cell lymphotropic virus types I and II by the immunofluorescence method
| Autor: | Dana Gallo, L M Penning, Carl V. Hanson |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Microbiology (medical)
Antigenicity viruses Clone (cell biology) Fluorescent Antibody Technique Biology Immunofluorescence Polymerase Chain Reaction Antigen immune system diseases hemic and lymphatic diseases medicine Humans HTLV-II Antigens Cell Line Transformed Human T-lymphotropic virus 1 medicine.diagnostic_test Human T-lymphotropic virus 2 Antibody titer virus diseases Virology Molecular biology HTLV-I Antibodies HTLV-II Antibodies Titer Evaluation Studies as Topic biology.protein Antibody HTLV-I Antigens Research Article |
| Zdroj: | Journal of Clinical Microbiology. 29:2345-2347 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.29.10.2345-2347.1991 |
| Popis: | We compared the sensitivities of the prototype human T-cell lymphotropic virus type I (HTLV-I)- and HTLV-II-transformed cell lines, MT2 and Mo-T, with that of an HTLV-II-infected cell line, clone 19, established in our laboratory, in the immunofluorescence (IF) test for detection of antibody to HTLV-I and HTLV-II. In addition, IF antibody titers with the three antigens were determined, and the results were compared with HTLV-I and HTLV-II typing by polymerase chain reaction (PCR). The MT2 cell line was more sensitive than the two HTLV-II cell lines for detecting HTLV-I antibody by IF, and clone 19 was more sensitive than Mo-T or MT2 for measuring HTLV-II antibody. In the titration study, the antigen that gave the highest titer correlated completely with the HTLV type determined by PCR, indicating that the relatively simple IF titration method can be used for differentiating HTLV-I and HTLV-II antibody in sera and plasmas. |
| Databáze: | OpenAIRE |
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