Core Alzheimer’s disease cerebrospinal fluid biomarker assays are not affected by aspiration or gravity drip extraction methods

Autor:	Hugo Vanderstichele, Steven J. Collins, Erik Stoops, Ralph N. Martins, Colin L. Masters, Qiao-Xin Li, Pierrick Bourgeat, James D. Doecke, Cindy Francois, Christopher Fowler, Stephanie R. Rainey-Smith, Victor L. Villemagne
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	0301 basic medicine Oncology medicine.medical_specialty Neurology Collection Cognitive Neuroscience Concordance tau Proteins Neurosciences. Biological psychiatry. Neuropsychiatry 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Alzheimer Disease Internal medicine medicine Aspartic Acid Endopeptidases Humans Cognitive Dysfunction Neurogranin RC346-429 Amyloid beta-Peptides medicine.diagnostic_test Lumbar puncture business.industry Research Australia Amyloid beta medicine.disease Peptide Fragments 030104 developmental biology Biomarker (medicine) Neurology (clinical) Tauopathy Neurology. Diseases of the nervous system Amyloid Precursor Protein Secretases Alzheimer's disease business Alzheimer’s disease 030217 neurology & neurosurgery Biomarkers RC321-571
Zdroj:	Alzheimer’s Research & Therapy, Vol 13, Iss 1, Pp 1-12 (2021) Alzheimer's Research & Therapy
ISSN:	1758-9193
Popis:	Background CSF biomarkers are well-established for routine clinical use, yet a paucity of comparative assessment exists regarding CSF extraction methods during lumbar puncture. Here, we compare in detail biomarker profiles in CSF extracted using either gravity drip or aspiration. Methods Biomarkers for β-amyloidopathy (Aβ1–42, Aβ1–40), tauopathy (total tau), or synapse pathology (BACE1, Neurogranin Trunc-p75, α-synuclein) were assessed between gravity or aspiration extraction methods in a sub-population of the Australian Imaging, Biomarkers and Lifestyle (AIBL) study (cognitively normal, N = 36; mild cognitive impairment, N = 8; Alzheimer’s disease, N = 6). Results High biomarker concordance between extraction methods was seen (concordance correlation > 0.85). Passing Bablock regression defined low beta coefficients indicating high scalability. Conclusions Levels of these commonly assessed CSF biomarkers are not influenced by extraction method. Results of this study should be incorporated into new consensus guidelines for CSF collection, storage, and analysis of biomarkers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe1764557e1ee4b144596f9f4e8185ea https://doaj.org/article/edbcb4cca5c24d489ba2634ab46b512a Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.