Flow cytometry and microscopic acridine orange test: relationship with standard semen analysis
| Autor: | De Yi Liu, Anwyn Apedaile, H.W. Gordon Baker, Stephanie A Johnston, Gary N. Clarke, C. Garrett |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Infertility endocrine system Cell Survival Semen Biology Semen analysis urologic and male genital diseases Sensitivity and Specificity Andrology Semen quality chemistry.chemical_compound fluids and secretions Reproductive biology medicine Humans Infertility Male Sperm motility Fluorescent Dyes Sperm Count medicine.diagnostic_test urogenital system Acridine orange Reproducibility of Results Obstetrics and Gynecology Flow Cytometry medicine.disease Spermatozoa Sperm Acridine Orange Reproductive Medicine chemistry Sperm Motility Developmental Biology |
| Zdroj: | Reproductive BioMedicine Online. 8:398-407 |
| ISSN: | 1472-6483 |
| DOI: | 10.1016/s1472-6483(10)60923-6 |
| Popis: | Improved prediction of male fertility requires advances in semen analysis. This study examined the reproducibility and independence of the flow cytometry acridine orange test (FCM-AOT) of sperm chromatin integrity as an assessment of semen quality. The study found that FCM-AOT results are not significantly affected by up to 6 h delay in semen preparation (n = 9) or contamination of semen with moderate concentrations of bacteria (10(8)/ml E. coli or Staph. epidermidis, n = 14). The variation of replicate measurements within samples was low (%Abnormal alpha(t): SD = 1.4, 95%CI = 4.6, n = 25) and different samples from the same men were mostly within the range of measurement error (n = 35). FCM-AOT variables, in particular %Abnormal alpha(t), displayed significant correlations with motility (r = -0.557), vitality (r = -0.469) and morphology (r = -0.464, n = 201), which are similar in magnitude to those existing between the standard semen variables. Surprisingly, no correlation was found between %Abnormal alpha(t) and the microscopic acridine orange test (M-AOT) (n = 185), suggesting the FCM results are sensitive to a different aspect of sperm quality. In summary, this study confirms that although not totally independent of standard semen analysis or the M-AOT, it is found to be a robust, sensitive and reproducible measure of semen quality, representative of the individual. |
| Databáze: | OpenAIRE |
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