Wolbachia-induced loss of male fertility is likely related to branch chain amino acid biosynthesis and iLvE in Laodelphax striatellus
| Autor: | Xing-Zhi Duan, Ary A. Hoffmann, Yan Guo, Jun-Tao Gong, Wen-Chao Zhu, Xiao-Yue Hong, Jia-Fei Ju, Yan-Kai Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Male media_common.quotation_subject Fertility 01 natural sciences Biochemistry Hemiptera 03 medical and health sciences Gene expression Copulation Testis Animals Amino Acids Molecular Biology Gene reproductive and urinary physiology media_common Oligonucleotide Array Sequence Analysis Genetics biology Embryo biochemical phenomena metabolism and nutrition biology.organism_classification Sperm 010602 entomology 030104 developmental biology Insect Science Host-Pathogen Interactions Insect Proteins Wolbachia Female Drosophila melanogaster Transcriptome Cytoplasmic incompatibility |
| Zdroj: | Insect biochemistry and molecular biology. 85 |
| ISSN: | 1879-0240 |
| Popis: | Wolbachia are endosymbionts that infect many species of arthropods and nematodes. Wolbachia-induced cytoplasmic incompatibility (CI) is the most common phenotype in affected hosts, involving embryonic lethality in crosses between Wolbachia-infected males and uninfected females. The molecular mechanisms underlying this phenomenon are currently unclear. Here we examine the molecular correlates of the Wolbachia infection in Laodelphax striatellus (Fallen), an important rice pest, where embryonic lethality is strong and almost complete. We compared the gene expression of 4-day-old Wolbachia-infected and uninfected L. striatellus testes to identify candidate genes for paternal-effect embryonic lethality induction. Based on microarray analysis, iLvE was the most down-regulated gene; this gene mediates branched-chain amino acid (BCAA) biosynthesis and participates in many processes related to reproductive performance. After knocking down iLvE by RNAi in uninfected male L. striatellus, male fertility was reduced, leading to a decrease in embryo hatching rates, but fertility was rescued in crosses between these males and Wolbachia-infected females. Removal of BCAA in chemically-defined diets of uninfected males also led to a loss of male fertility. Low amino acid nutrition may enhance exposure time of sperm to Wolbachia in the testes to affect adult reproduction in L. striatellus by reducing the number of sperm transferred per mating by males. These results indicate that Wolbachia may decrease male fertility in L. striatellus by acting on iLvE, a key factor of BCAA biosynthesis, and delaying sperm maturation. |
| Databáze: | OpenAIRE |
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