Incidence of nickel-induced sister-chromatid exchange
| Autor: | Sheldon M. Newman, Loys J. Nunez, Robert L. Summitt |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Genetics
education.field_of_study Dose-Response Relationship Drug Incidence (epidemiology) Population chemistry.chemical_element Karyotype Sister chromatid exchange Biology Toxicology Molecular biology Nickel chemistry Chromosomes Human Humans Crossing Over Genetic Lymphocytes Nickelous Chloride education Sister Chromatid Exchange Cells Cultured Carcinogen Mutagens |
| Zdroj: | Mutation Research/Genetic Toxicology. 101:67-75 |
| ISSN: | 0165-1218 |
| DOI: | 10.1016/0165-1218(82)90166-5 |
| Popis: | Nickel is a recognized carcinogen to which the general population is being increasingly exposed. A nickel-induced alteration in the incidence of sister-chromatid exchanges in cultured lymphocytes has been investigated. Human lymphocytes from a single donor were cultured in media containing various concentrations of nickelous chloride from 10 −6 to 10 −3 M and a control culture containing no added nickel. A significantly increased incidence of exchanges over background was induced at 1.19 × 10 −4 M as indicated by a Student's t -test. A concentration/response curve was generated up to 5 × 10 −4 M where the nickel concentration was lethal to the cells. The nickel response was analyzed by a chi-square test for any variation in location of activity across the karyotype and no differences were detected. The background incidence did vary from a uniform distribution across the karyotype with increased incidences in chromosomes 1 and 2 and those of the B group, and decreased incidences in the E, F, and G groups. |
| Databáze: | OpenAIRE |
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