Preliminary evidence of the association between DNAm and orbital volumetry in GO
| Autor: | Yun-Fu Liu, Zhong Xin, Yi-Lin Yang, Zhong-Yu Yan, Xiu Tuo, Lin Hua, Ting-Ting Shi, Ya-Fen Hu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Candidate gene
Endocrinology Diabetes and Metabolism Bisulfite sequencing 030209 endocrinology & metabolism Trab lcsh:Diseases of the endocrine glands. Clinical endocrinology 03 medical and health sciences 0302 clinical medicine Endocrinology Internal Medicine Medicine Gene orbit lcsh:RC648-665 DNA methylation business.industry Research dNaM Methylation Molecular biology graves’ orbitopathy extraocular muscles 030221 ophthalmology & optometry Multiple linear regression analysis business |
| Zdroj: | Endocrine Connections Endocrine Connections, Vol 9, Iss 7, Pp 617-626 (2020) |
| ISSN: | 2049-3614 |
| DOI: | 10.1530/ec-20-0147 |
| Popis: | Background The pathogenesis underlying the alterations of orbital architecture in Graves’ orbitopathy (GO) is not yet fully understood. The present study aimed to investigate the association of DNA methylation in peripheral blood and orbital volumetry in Chinese patients with GO. Methods A total of 35 GO subjects (70 orbits) were subjected to CT scan. The total cross-sectional area of the extraocular muscles (orbital muscles, OM), total orbit area (TOA), and the exophthalmometry were measured and OM/TOA ratio was calculated. Targeted bisulfite sequencing was performed on seven candidate genes. Results No significant correlation was established between the DNA methylation levels of these genes and exophthalmometry. The MBP methylation level was found to be correlated with OM/TOA ratio (P < 0.05). Multiple linear regression analysis on parameters including age, sex, TRAb, duration of GO, and DNA methylation levels of seven genes with OM/TOA ratio confirmed that MBP and OM/TOA ratio had a significant correlation (P < 0.05). The partial least squares analysis showed that the top three genes with the highest loadings were MBP, BOLL, and BECN1 and that OM/TOA ratio affected the DNA methylation block than exophthalmometry. Conclusions This study provided preliminary evidence that MBP is a potential gene associated with OM enlargement in GO patients according to the combination of DNA methylation sequencing and orbital CT measurement. |
| Databáze: | OpenAIRE |
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