A single-cell analysis of the molecular lineage of chordate embryogenesis
| Autor: | Kaoru Imai, Guilin Wang, Yichi Xu, Zhirong Bao, Weiyang Shi, Bo Dong, Tengjiao Zhang, Teng Fei, Tianwei Yu, Yutaka Satou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Brachyury
Cell type animal structures Lineage (genetic) Ciona savignyi genetic processes Notochord Cell fate determination Biology Mice 03 medical and health sciences 0302 clinical medicine Single-cell analysis medicine Animals natural sciences Cell Lineage Research Articles 030304 developmental biology 0303 health sciences Multidisciplinary fungi SciAdv r-articles Gene Expression Regulation Developmental Embryo Cell cycle biology.organism_classification Embryonic stem cell Ciona intestinalis Cell biology Ciona Fibroblast Growth Factors Multicellular organism medicine.anatomical_structure embryonic structures Single-Cell Analysis 030217 neurology & neurosurgery Research Article Developmental Biology |
| Zdroj: | Science Advances |
| DOI: | 10.1101/2020.03.02.966440 |
| Popis: | scRNA-seq of an invariant cell lineage reveals insights into asymmetric cell division, FGF signaling, and notochord specification. Progressive unfolding of gene expression cascades underlies diverse embryonic lineage development. Here, we report a single-cell RNA sequencing analysis of the complete and invariant embryonic cell lineage of the tunicate Ciona savignyi from fertilization to the onset of gastrulation. We reconstructed a developmental landscape of 47 cell types over eight cell cycles in the wild-type embryo and identified eight fate transformations upon fibroblast growth factor (FGF) inhibition. For most FGF-dependent asymmetric cell divisions, the bipotent mother cell displays the gene signature of the default daughter fate. In convergent differentiation of the two notochord lineages, we identified additional gene pathways parallel to the master regulator T/Brachyury. Last, we showed that the defined Ciona cell types can be matched to E6.5-E8.5 stage mouse cell types and display conserved expression of limited number of transcription factors. This study provides a high-resolution single-cell dataset to understand chordate early embryogenesis and cell lineage differentiation. |
| Databáze: | OpenAIRE |
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