Analysis and bioequivalency study on two tablet formulations of co–trimoxazole
| Autor: | F. F. Amari, H. N. Alkaysi, A. M. Gharaibeh, E. Sallam, M. Sheikh Salem, M. S. Shubair |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Pharmacology Chromatography Ethyl acetate Biological Availability Reproducibility of Results Sensitivity and Specificity High-performance liquid chromatography Dosage form Bioavailability chemistry.chemical_compound Therapeutic Equivalency chemistry Trimethoprim Sulfamethoxazole Drug Combination medicine Humans Hydroxide Pharmacology (medical) Ammonium acetate Chromatography High Pressure Liquid Tablets Antibacterial agent Tetroxoprim medicine.drug |
| Zdroj: | Journal of Clinical Pharmacy and Therapeutics. 18:109-114 |
| ISSN: | 1365-2710 0269-4727 |
| DOI: | 10.1111/j.1365-2710.1993.tb00576.x |
| Popis: | This paper describes an analytical procedure for simultaneous quantification of trimethoprim (TMP) and sulphamethoxazole (SMZ) in serum. Serum samples were rendered alkaline with glycine-sodium hydroxide buffer (pH 9.1) and extracted with ethyl acetate. The reconstituted sample was analysed using reversed-phase C-18 column high-performance chromatography. The mobile phase consisted of 75% triethyl ammonium acetate buffer, 20% methanol and 5% acetonitrile. The analytes were monitored at 289 nm and tetroxoprim was used as an internal standard. The maximum values for intra-day coefficients of variation (CV) for TMP-and SMZ were 5.7 and 2.1%, respectively. Interday CV values were 6.4 and 2.2%, respectively. The method was used to compare the bioavailability of two tablet formulations in terms of their pharmacokinetic parameters following oral administration of the tablets to 18 volunteers. |
| Databáze: | OpenAIRE |
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