Flavonoid supplementation affects the expression of genes involved in cell wall formation and lignification metabolism and increases sugar content and saccharification in the fast-growing eucalyptus hybrid E. urophylla x E. grandis
| Autor: | João Paulo L. Franco Cairo, Piotr A. Mieczkowski, Jorge Lepikson-Neto, Gonçalo Amarante Guimarães Pereira, Wesley Leoricy Marques, Ana Carolina Deckmann, Marcela Salazar, Leandro Costa do Nascimento, Paulo José Pereira Lima Teixeira, Fabio M. Squina, Eduardo Leal Oliveira Camargo |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Sucrose
Flavonoid Syringyl/guaiacyl ratio Down-Regulation Plant Science Biology Stress Lignin Trees chemistry.chemical_compound Cell Wall Gene Expression Regulation Plant Phenylpropanoid metabolism Botany Hardwood Cultivar Sugar Hybrid chemistry.chemical_classification Flavonoids Eucalyptus Hydrolysis food and beverages Wood chemistry Carbohydrate Metabolism Gene expression Research Article |
| Zdroj: | BMC Plant Biology |
| ISSN: | 1471-2229 |
| Popis: | Background Eucalyptus species are the most widely planted hardwood species in the world and are renowned for their rapid growth and adaptability. In Brazil, one of the most widely grown Eucalyptus cultivars is the fast-growing Eucalyptus urophylla x Eucalyptus grandis hybrid. In a previous study, we described a chemical characterization of these hybrids when subjected to flavonoid supplementation on 2 distinct timetables, and our results revealed marked differences between the wood composition of the treated and untreated trees. Results In this work, we report the transcriptional responses occurring in these trees that may be related to the observed chemical differences. Gene expression was analysed through mRNA-sequencing, and notably, compared to control trees, the treated trees display differential down-regulation of cell wall formation pathways such as phenylpropanoid metabolism as well as differential expression of genes involved in sucrose, starch and minor CHO metabolism and genes that play a role in several stress and environmental responses. We also performed enzymatic hydrolysis of wood samples from the different treatments, and the results indicated higher sugar contents and glucose yields in the flavonoid-treated plants. Conclusions Our results further illustrate the potential use of flavonoids as a nutritional complement for modifying Eucalyptus wood, since, supplementation with flavonoids alters its chemical composition, gene expression and increases saccharification probably as part of a stress response. Electronic supplementary material The online version of this article (doi:10.1186/s12870-014-0301-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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