In vitro SOD-like activity of mono- and di-copper complexes with a phosphonate substituted SALAN-type ligand
| Autor: | Łukasz Szyrwiel, Paweł Tokarz, László Wojnárovits, Wolfgang Caliebe, Zbigniew Szewczuk, Krisztina J. Kovács, Vadim Murzin, Mari Shimura, József S. Pap, Justyna Brasuń, Inna Szekacs, Aneta Paluch, Robert Horvath, Tünde Tóth, Adam Kubas |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell Survival Molecular Conformation chemistry.chemical_element Disproportionation Ligands Toxicology Redox Medicinal chemistry Antioxidants Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine 3T3-L1 Cells Organometallic Compounds Animals Molecule Cells Cultured Superoxide Dismutase Chemistry Ligand General Medicine Phosphonate Copper Small molecule 030104 developmental biology 030220 oncology & carcinogenesis Radiolysis Oxidation-Reduction |
| Zdroj: | Chemico-Biological Interactions. 306:78-88 |
| ISSN: | 0009-2797 |
| Popis: | SALEN- and SALAN-based complexes with catalytically active metal centers are very promising small molecules to be utilized as part of antioxidant therapies. Here we discuss a modified SALAN-type molecule armed with two phosphonate groups that significantly increase its water solubility and aid to furnish mono- or dinuclear complexes with Cu2+ ions. The regulation of the SOD-mimicking (i.e., catalytic) disproportionation reaction of the superoxide radical anion (O2•−) at pH ~7.5 could be achieved by adjusting the metal-to-ligand stoichiometry as confirmed by McCord-Fridovich and pulse radiolysis tests. The higher antioxidant activity of the dicopper complex can be explained by the better access of O2•− to the copper centers and their more positive Cu(II)/Cu(I) redox potential. Simultaneously the analysis of in vitro effect on cells morphology indicates that cytotoxicity is also affected by the metal-to-ligand ratio, however, the active complex molecules do not show notable cytotoxicity that, together with the observed SOD-like activities, makes them potential candidates for antioxidant therapies. |
| Databáze: | OpenAIRE |
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