Drug-Loadable Calcium Alginate Hydrogel System for Use in Oral Bone Tissue Repair

Autor: Satoshi Komasa, Yepo Hou, Xue Yanxiang, Joji Okazaki, Bingyu Jin, Luyuan Chen, Renze Shen, Jie Gao
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Calcium alginate
Bone Regeneration
02 engineering and technology
Mandible
Bone tissue
chemistry.chemical_compound
Polylactic acid
Glucuronic Acid
drug-loadable system
Spectroscopy
Cells
Cultured
Tissue Scaffolds
calcium alginate hydrogels
Hexuronic Acids
Hydrogels
General Medicine
Anatomy
021001 nanoscience & nanotechnology
Computer Science Applications
medicine.anatomical_structure
Self-healing hydrogels
human periodontal ligament cells
Female
Rabbits
0210 nano-technology
Stromal cell
Biocompatibility
Alginates
Periodontal Ligament
macromolecular substances
Mesenchymal Stem Cell Transplantation
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
biocompatibility
stomatognathic system
medicine
Periodontal fiber
Animals
Humans
tissue engineering
Physical and Theoretical Chemistry
Molecular Biology
Tissue Engineering
Organic Chemistry
technology
industry
and agriculture
Mesenchymal Stem Cells
030104 developmental biology
chemistry
Bone marrow
Biomedical engineering
Zdroj: International Journal of Molecular Sciences; Volume 18; Issue 5; Pages: 989
International Journal of Molecular Sciences
ISSN: 1422-0067
DOI: 10.3390/ijms18050989
Popis: This study developed a drug-loadable hydrogel system with high plasticity and favorable biological properties to enhance oral bone tissue regeneration. Hydrogels of different calcium alginate concentrations were prepared. Their swelling ratio, degradation time, and bovine serum albumin (BSA) release rate were measured. Human periodontal ligament cells (hPDLCs) and bone marrow stromal cells (BMSCs) were cultured with both calcium alginate hydrogels and polylactic acid (PLA), and then we examined the proliferation of cells. Inflammatory-related factor gene expressions of hPDLCs and osteogenesis-related gene expressions of BMSCs were observed. Materials were implanted into the subcutaneous tissue of rabbits to determine the biosecurity properties of the materials. The materials were also implanted in mandibular bone defects and then scanned using micro-CT. The calcium alginate hydrogels caused less inflammation than the PLA. The number of mineralized nodules and the expression of osteoblast-related genes were significantly higher in the hydrogel group compared with the control group. When the materials were implanted in subcutaneous tissue, materials showed favorable biocompatibility. The calcium alginate hydrogels had superior osteoinductive bone ability to the PLA. The drug-loadable calcium alginate hydrogel system is a potential bone defect reparation material for clinical dental application.
Databáze: OpenAIRE
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