Protein expression of KIT, BRAF, GNA11, GNAQ and RASSF1 in feline diffuse iris melanomas
| Autor: | S. Kummer, A. Fuchs-Baumgartinger, Barbara Nell, J.G. Rushton, M. Korb, Alexander Tichy, Ursula Reichart |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Proto-Oncogene Proteins B-raf
0301 basic medicine Fluorescent Antibody Technique Cat Diseases Immunofluorescence Proto-Oncogene Mas Receptor tyrosine kinase 03 medical and health sciences 0302 clinical medicine Gene expression medicine Animals Iris Neoplasms Melanoma neoplasms General Veterinary GNA11 biology Oncogene medicine.diagnostic_test Kinase Tumor Suppressor Proteins Iris melanoma GTP-Binding Protein alpha Subunits Proto-Oncogene Proteins c-kit 030104 developmental biology Protein Biosynthesis 030220 oncology & carcinogenesis Cats biology.protein Cancer research GTP-Binding Protein alpha Subunits Gq-G11 Female Animal Science and Zoology GNAQ |
| Zdroj: | The Veterinary Journal. 249:33-40 |
| ISSN: | 1090-0233 |
| DOI: | 10.1016/j.tvjl.2019.04.008 |
| Popis: | Feline iris melanoma, the most common feline intraocular tumour, has a reported metastatic rate of 19-63%. However, there is a lack of knowledge about its molecular biology. Previous studies have reported that feline iris melanomas do not harbour mutations comparable to common mutations found in their human counterpart. Nevertheless, there are differences in the gene expression patterns. The aim of this study was to investigate the protein expression of B-RAF oncogene serine/threonine kinase (BRAF), G protein subunit alpha q (GNAQ) and 11 (GNA11), KIT proto-oncogene receptor tyrosine kinase (KIT), and Ras association family member 1 (RASSF1) in feline iris melanomas. Fifty-seven formalin-fixed paraffin embedded (FFPE) iris melanomas and 25 FFPE eyes without ocular abnormalities were stained with antibodies against the respective proteins using immunofluorescence. Averaged pixel intensities/μm2 and percentage of stained area from total tissue area were measured and the results were compared. Compared to the control group, iris melanomas showed overexpression of BRAF, GNAQ, GNA11 and KIT. The higher expression of BRAF, GNAQ, GNA11 and KIT in feline iris melanomas suggest that these proteins may play a key role in the development of feline iris melanomas and KIT may present a possible target for future therapies in cats with feline iris melanomas. |
| Databáze: | OpenAIRE |
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