Polymorphisms at the IL17A and IL17RA Genes are Associated with Prognosis of Papillary Thyroid Carcinoma
| Autor: | Bruna Cristina Bertol, Marcos Gonzaga dos Santos, Gustavo Martelli-Palomino, Jéssica Nayara Góes de Araújo, Nathalie Lobo de Figueiredo Feitosa, Vivian Nogueira Silbiger, Kleyton Thiago Costa de Carvalho, Léa Maria Zanini Maciel, Eduardo Antônio Donadi, Norma Lucena-Silva, Janaina Cristiana de Oliveira Crispim Freitas |
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| Rok vydání: | 2022 |
| Předmět: |
Receptors
Interleukin-17 Genotype endocrine system diseases business.industry Interleukin-17 Single-nucleotide polymorphism General Medicine Prognosis medicine.disease Polymorphism Single Nucleotide Thyroid carcinoma Thyroid Cancer Papillary Case-Control Studies medicine Cancer research Humans SNP Genetic Predisposition to Disease Thyroid Neoplasms IL17A Restriction fragment length polymorphism Allele business Thyroid cancer |
| Zdroj: | Archives of Medical Research. 53:163-169 |
| ISSN: | 0188-4409 |
| DOI: | 10.1016/j.arcmed.2021.07.004 |
| Popis: | Background Interleukin (IL)-17A has a dual role in tumor immunity, promotes anti-tumor responses and facilitates angiogenesis by interacting with IL-17 receptor A (IL-17RA). Although IL-17A has been associated with the pathogenesis of papillary thyroid carcinoma (PTC), the nucleotide variability at the IL17A and IL17RA genes is still poorly characterized. Aim To assess the contribution of the IL17A (-197 G >A, rs2275913) and IL17RA (-947 A >G, rs4819554) single nucleotide polymorphisms (SNP) on the development and progression of PTC and on IL-17 plasma levels. Methods We studied 188 PTC patients and 170 healthy controls. SNPs were identified using PCR-amplified DNA and restriction fragment length polymorphism (RFLP) techniques. Plasma levels of IL-17A was evaluated in 83 PTC patients using ELISA. Statistical analyses were performed to evaluate the associations between SNPs and clinicohistopathological features of PTC and IL-17A levels. Results No significant difference was observed regarding the allele and genotype distributions of both SNPs between PTC patients and controls. The IL17A GA was associated with poor biochemical and structural incomplete response to therapy, whereas no influence over the IL-17A expression was observed. The IL17RA AG was significantly associated with small-sized tumors, initial tumor stage at diagnosis and better response to therapy. Conclusions The IL17A SNP may predict an aggressive manifestation of PTC, whereas the IL17RA SNP was associated with a more favorable clinical outcome. |
| Databáze: | OpenAIRE |
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