Identification of glucuronan lyase from a mutant strain of Rhizobium meliloti
| Autor: | Jean-Noël Barbotin, Patrice Pheulpin, Josiane Courtois, Bernard Courtois, Emmanuel Petit, Philippe Michaud, J. P. Seguin, Alain Heyraud |
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| Rok vydání: | 1997 |
| Předmět: |
Magnetic Resonance Spectroscopy
Carbohydrates Polysaccharide Biochemistry Structural Biology Glucuronan lyase Polysaccharides Molecular Biology Polysaccharide-Lyases chemistry.chemical_classification Chromatography biology Polysaccharides Bacterial food and beverages Substrate (chemistry) Acetylation General Medicine biology.organism_classification Lyase Enzyme assay Enzyme chemistry Mutation biology.protein Rhizobium Carbohydrate Metabolism Fermentation Sinorhizobium meliloti |
| Zdroj: | International journal of biological macromolecules. 21(1-2) |
| ISSN: | 0141-8130 |
| Popis: | The Rhizobium meliloti M5N1CS (NCIMB 40472) mutant strain wich induces nodule formation on alfalfa roots, produces a (1→4)-β- d -glucuronan partially acetylated. During fermentation under specific conditions, the molecular weight of the polymer decrease, the presence of polysaccharide degrading enzyme was suspected. A glucuronan lyase was identified, this new bacterial lyase produces d.p. 4 oligoglucuronans, substituents (acetates) present on the substrate reduced the enzyme activity. |
| Databáze: | OpenAIRE |
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