Human multidrug-resistant Mycobacterium bovis infection in Mexico

Autor: Armando Martinez-Guarneros, Gabriela A. Gonzalez-Macal, Jorge A. Gonzalez-y-Merchand, David Couvin, Nalin Rastogi, Gilberto Vaughan, Alejandro Escobar-Gutiérrez, Carlos A. Vazquez-Chacon, Sandra Rivera-Gutierrez, Raquel Muñiz-Salazar, Juan J. De-la-Cruz López, Livia Maria Gonçalves Rossi, Adriana Gomez-Bustamante
Přispěvatelé: Escuela Nacional de Ciencias Bilogicas, Instituto Politecnico Nacional [Mexico] (IPN), Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Mexico city, Unité de la Tuberculose et des Mycobactéries - WHO Supranational TB Reference Laboratory, Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Laboratorio Estatal de Salud Pública, Secretaría de Salud, Chiapas, Centro Mesoamericano de Estudios en Salud Pública y Desastres, Escuela de Ciencias de la Salud, Universidad Autónoma de Baja California (UABC), This work was partially supported by CONACYT-SALUD-2010-C01-139579 and as part of the 7th Framework Program, European Commission, contract No. HEALTH-F3-2008-200999, and by grants CB-2010-156347, CONACYT and SIP20140970, IPN, Mexico.
Rok vydání: 2015
Předmět:
Zdroj: Tuberculosis
Tuberculosis, Elsevier, 2015, 95 (6), pp.802-9. ⟨10.1016/j.tube.2015.07.010⟩
ISSN: 1873-281X
1472-9792
DOI: 10.1016/j.tube.2015.07.010⟩
Popis: International audience; Here, we describe the molecular characterization of six human Mycobacterium bovis clinical isolates, including three multidrug resistant (MDR) strains, collected in Mexico through the National Survey on Tuberculosis Drug Resistance (ENTB-2008), a nationally representative survey conducted during 2008-2009 in nine states with a stratified cluster sampling design. The genetic background of bovine M. bovis strains identified in three different states of Mexico was studied in parallel to assess molecular relatedness of bovine and human strains. Additionally, resistance to first and second line anti-tuberculosis (TB) drugs and molecular identification of mutations conferring drug resistance was also performed. All strains were characterized by spoligotyping and 24-loci MIRU-VNTRs, and analyzed using the SITVIT2 (n = 112,000 strains) and SITVITBovis (n = 25,000 strains) proprietary databases of Institut Pasteur de la Guadeloupe. Furthermore, data from this study (n = 55 isolates), were also compared with genotypes recorded for M. bovis from USA (n = 203), Argentina (n = 726), as well as other isolates from Mexico (independent from the present study; n = 147), to determine any evidence for genetic relatedness between circulating M. bovis strains. The results showed that all human M. bovis cases were not genetically related between them or to any bovine strain. Interestingly, a high degree of genetic variability was observed among bovine strains. Several autochthonous and presumably imported strains were identified. The emergence of drug-resistant M. bovis is an important public health problem that jeopardizes the success of TB control programs in the region.
Databáze: OpenAIRE
Externí odkaz: