Remote Magnetic Nanoparticle Manipulation Enables the Dynamic Patterning of Cardiac Tissues

Autor: Cesare M. Terracciano, Simone Zonetti, Limor Zwi-Dantsis, Lucia Massi, Brian Wang, Molly M. Stevens, Arianna Ferrini, Daniel J. Stuckey, Camille Marijon
Přispěvatelé: Commission of the European Communities, Medical Research Council (MRC), British Heart Foundation, Wellcome Trust, Kusuma Trust UK
Rok vydání: 2019
Předmět:
Technology
Chemistry
Multidisciplinary
Nanoparticle
Biocompatible Materials
02 engineering and technology
01 natural sciences
Regenerative medicine
09 Engineering
chemistry.chemical_compound
General Materials Science
Myocytes
Cardiac
Cellular organization
Magnetite Nanoparticles
ENGINEERED HEART-TISSUE
02 Physical Sciences
patterning
Tissue Scaffolds
Chemistry
Physical
CHIP
Physics
Hydrogels
Equipment Design
MUSCLE
021001 nanoscience & nanotechnology
Soft materials
3. Good health
Chemistry
Physics
Condensed Matter
Mechanics of Materials
Self-healing hydrogels
Physical Sciences
Science & Technology - Other Topics
Collagen
0210 nano-technology
03 Chemical Sciences
Iron oxide nanoparticles
magnetic nanoparticles
Materials science
Materials Science
Materials Science
Multidisciplinary
cellular organization
010402 general chemistry
Article
Physics
Applied
Cell Line
Humans
Nanoscience & Nanotechnology
FIELD
Science & Technology
Tissue Engineering
Mechanical Engineering
PLATFORM
0104 chemical sciences
Magnetic Fields
chemistry
CELLS
Magnetic nanoparticles
cardiac tissues
MYOCARDIUM
Biomedical engineering
Zdroj: Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Popis: The ability to manipulate cellular organization within soft materials has important potential in biomedicine and regenerative medicine; however, it often requires complex fabrication procedures. Here, a simple, cost-effective, and one-step approach that enables the control of cell orientation within 3D collagen hydrogels is developed to dynamically create various tailored microstructures of cardiac tissues. This is achieved by incorporating iron oxide nanoparticles into human cardiomyocytes and applying a short-term external magnetic field to orient the cells along the applied field to impart different shapes without any mechanical support. The patterned constructs are viable and functional, can be detected by T2 *-weighted magnetic resonance imaging, and induce no alteration to normal cardiac function after grafting onto rat hearts. This strategy paves the way to creating customized, macroscale, 3D tissue constructs with various cell-types for therapeutic and bioengineering applications, as well as providing powerful models for investigating tissue behavior.
Databáze: OpenAIRE
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