Efficacy of a Hypotonic Treatment for Peritoneal Dissemination from Gastric Cancer Cells: AnIn VivoEvaluation
| Autor: | Hiroki Shimizu, Toshiyuki Kosuga, Yoshinori Marunaka, Maki Kitagawa, Yoshito Nako, Atsushi Shiozaki, Shuhei Komatsu, Kazuma Okamoto, Hitoshi Fujiwara, Kenichi Takemoto, Hirotaka Konishi, Shingo Nakashima, Eigo Otsuji, Daisuke Ichikawa |
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| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Article Subject lcsh:Medicine Abdominal cavity Sodium Chloride General Biochemistry Genetics and Molecular Biology Mice Peritoneal Neoplasm Stomach Neoplasms In vivo Cell Line Tumor medicine Animals Humans Peritoneal Neoplasms General Immunology and Microbiology business.industry lcsh:R Water General Medicine Xenograft Model Antitumor Assays In vitro medicine.anatomical_structure Hypotonic Solutions Cell culture Toxicity Cancer cell Tonicity business Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2014 (2014) |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2014/707089 |
| Popis: | The aim of the present study was to determine the efficacy of a hypotonic treatment for peritoneal dissemination from gastric cancer cells using anin vivomodel. We firstly evaluated the toxicity of a peritoneal injection of distilled water (DW) (2 mL for 3 days) in mice. Macroscopic and microscopic examinations revealed that the peritoneal injection of DW did not severely damage the abdominal organs of these mice. MKN45 gastric cancer cells preincubated with NaCl buffer or DW for 20 minutesin vitrowere then intraperitoneally injected into nude mice, and the development of dissemination nodules was analyzed. The total number, weight, and volume of the dissemination nodules were significantly decreased by the DW preincubation. We then determined whether the peritoneal injection of DW inhibited the establishment of peritoneal dissemination. After a peritoneal injection of MKN45 cells into nude mice, NaCl buffer or DW was injected into the abdominal cavity for 3 days. The total volume of dissemination nodules was significantly lower in DW-injected mice than in NaCl-injected mice. In conclusion, we demonstrated the safeness of a peritoneal injection of DW. Furthermore, the development of dissemination nodules from gastric cancer cells was prevented by a preincubation with or peritoneal injection of DW. |
| Databáze: | OpenAIRE |
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