Coculturing of Mosquito‐Microbiome Bacteria Promotes Heme Degradation in Elizabethkingia anophelis
| Autor: | Emily R. Derbyshire, Jack G. Ganley, Hannah K. D'Ambrosio, Meg Shieh |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
food.ingredient
Elizabethkingia Heme 010402 general chemistry medicine.disease_cause 01 natural sciences Biochemistry Microbiology chemistry.chemical_compound food Bacterial Proteins Anopheles medicine Animals Microbiome Molecular Biology Phylogeny Biliverdin Virulence biology 010405 organic chemistry Microbiota Organic Chemistry Pseudomonas Sequence Analysis DNA biology.organism_classification Coculture Techniques 0104 chemical sciences chemistry Metagenomics Elizabethkingia anophelis Molecular Medicine Flavobacteriaceae Genome Bacterial Bacteria |
| Zdroj: | ChemBioChem. 21:1279-1284 |
| ISSN: | 1439-7633 1439-4227 |
| Popis: | Anopheles mosquito microbiomes are intriguing ecological niches. Within the gut, microbes adapt to oxidative stress due to heme and iron after blood meals. Although metagenomic sequencing has illuminated spatial and temporal fluxes of microbiome populations, limited data exist on microbial growth dynamics. Here, we analyze growth interactions between a dominant microbiome species, Elizabethkingia anophelis, and other Anopheles-associated bacteria. We find E. anophelis inhibits a Pseudomonas sp. via an antimicrobial-independent mechanism and observe biliverdins, heme degradation products, upregulated in cocultures. Purification and characterization of E. anophelis HemS demonstrates heme degradation, and we observe hemS expression is upregulated when cocultured with Pseudomonas sp. This study reveals a competitive microbial interaction between mosquito-associated bacteria and characterizes the stimulation of heme degradation in E. anophelis when grown with Pseudomonas sp. |
| Databáze: | OpenAIRE |
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