Altered Sigma-1 Receptor Expression in Two Animal Models of Cognitive Impairment
| Autor: | Csaba Nyakas, Philip H. Elsinga, Paul G.M. Luiten, Aren van Waarde, Kiichi Ishiwata, Krisztina Marosi, Nisha Kuzhuppilly Ramakrishnan, Chantal Kwizera, Rudi Dierckx |
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| Přispěvatelé: | Molecular Neuroscience and Ageing Research (MOLAR), Guided Treatment in Optimal Selected Cancer Patients (GUTS), Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE) |
| Rok vydání: | 2014 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Receptor expression Blotting Western Novel object recognition Rapid eye movement sleep Sigma-1 receptor RAT-BRAIN Nucleus basalis Choline Internal medicine Reaction Time SELECTIVE CHOLINERGIC LESION medicine Animals Receptors sigma Tissue Distribution NUCLEUS BASALIS MAGNOCELLULARIS Radiology Nuclear Medicine and imaging Rats Wistar DEPRIVATION Receptor Cholinergic lesion REM sleep deprivation UNFOLDED PROTEIN RESPONSE business.industry Somatosensory Cortex microPET Passive avoidance Pons Disease Models Animal Kinetics MICE Sleep deprivation Endocrinology [C-11]SA4503 Oncology REM-SLEEP Anesthesia DONEPEZIL Sleep Deprivation Cholinergic medicine.symptom Cognition Disorders business INDUCED CELL-DEATH EYE-MOVEMENT SLEEP |
| Zdroj: | Molecular Imaging and Biology, 17(2), 231-238. SPRINGER |
| ISSN: | 1860-2002 1536-1632 |
| DOI: | 10.1007/s11307-014-0780-x |
| Popis: | PURPOSE: Sigma-1 receptors are involved in learning and memory processes. We assessed sigma-1 receptor expression and memory function in two animal models of cognitive impairment.PROCEDURES: Male Wistar-Hannover rats were either lesioned by unilateral injection of N-methyl-D-aspartic acid in the nucleus basalis, or deprived of rapid eye movement sleep for 48 h, using the modified multiple platform method. Sigma-1 receptor expression was examined with the positron emission tomography radiotracer [(11)C]SA4503, immunohistochemistry, and Western blotting.RESULTS: Cortical tracer uptake after 1 week was not significantly affected by lesioning. Immunohistochemistry revealed moderate increases of sigma-1 receptors at bregma level -2.8, in parietal cortex layer V of the lesioned hemisphere. Sleep deprivation lowered passive avoidance test scores and reduced [(11)C]SA4503 accumulation and sigma-1 receptor expression in pons.CONCLUSIONS: Cholinergic lesioning causes an increase of sigma-1 receptor expression in a small cortical area which may be neuroprotective. Sleep deprivation decreases receptor expression in midbrain and pons. |
| Databáze: | OpenAIRE |
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