Acylated ascorbate stimulates collagen synthesis in cultured human foreskin fibroblasts at lower doses than does ascorbic acid
| Autor: | Marcel E. Nimni, Adl Jonas, Nino Sorgente, Natasha Perelman, Ishak Neeman, Gennady Rosenblat, Sissi Gal-Or, Ella Katzir |
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| Rok vydání: | 1998 |
| Předmět: |
Time Factors
Acylation Ascorbic Acid Deferoxamine Biochemistry Antioxidants Foreskin Rheumatology medicine Cytotoxic T cell Humans Orthopedics and Sports Medicine Molecular Biology Cells Cultured Skin Dose-Response Relationship Drug Chemistry Serum Albumin Bovine Cell Biology DNA Fibroblasts Ascorbic acid Hydroquinones medicine.anatomical_structure Ascorbate Oxidase lipids (amino acids peptides and proteins) Collagen Oxidation-Reduction |
| Zdroj: | Connective tissue research. 37(3-4) |
| ISSN: | 0300-8207 |
| Popis: | Acylated derivatives of ascorbic acid were found to be active in a number of biochemical and physiological processes. In the present study we investigated the effects of 6-O-palmitoyl ascorbate on collagen synthesis by cultured foreskin human fibroblasts. Our observations indicate a marked stimulatory effect on collagen synthesis by 6-O-palmitoyl ascorbate in the concentration range of 5-20 microM, while the synthesis stimulated by ascorbic acid was maximal at concentrations of 20-100 microM. Cells treated with 10 microM palmitoyl ascorbate for 36 h exhibited a production of collagen threefold greater than those in the presence of 10 microM ascorbic acid, and it was about the same as in cells treated with 100 microM ascorbic acid. By 48 h differences were not significant. Acylated ascorbate impaired vitality of the treated fibroblasts at concentrations exceeding 20 microM in media supplemented with 0.5% FCS. However, most of the cytotoxic effect was neutralized by FCS at a concentration of 10%. The resistance of acylated ascorbate against oxidative degradation as well as the role of free radicals in the modulation of collagen synthesis by ascorbic acid and by its derivatives is discussed. |
| Databáze: | OpenAIRE |
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