Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus)
Autor: | Carolina H. S. Borges, José M. Yáñez, Ricardo Utsunomia, Ross D. Houston, Carolina Peñaloza, Vito A. Mastrochirico-Filho, Diogo Teruo Hashimoto, Fabiana Pilarski, Roberto Carvalheiro, Milena V. Freitas, Alejandro P. Gutierrez, Raquel B. Ariede |
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Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal Rural Do Rio de Janeiro, National Council for Scientific and Technological Development (CNPq), University of Edinburgh, Universidad de Chile |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Candidate gene
lcsh:QH426-470 Genetic Linkage lcsh:Biotechnology Quantitative Trait Loci Single-nucleotide polymorphism Genome-wide association study Quantitative trait locus Biology Polymorphism Single Nucleotide Fish Diseases 03 medical and health sciences Fish farming lcsh:TP248.13-248.65 Genetic variation Genetics Animals Disease outbreaks Disease Resistance 030304 developmental biology 0303 health sciences 0402 animal and dairy science Resistance QTLs 04 agricultural and veterinary sciences Marker-assisted selection Heritability 040201 dairy & animal science Motile Aeromonas septicemia Genetic architecture Aeromonas hydrophila lcsh:Genetics Characiformes Gram-Negative Bacterial Infections Research Article Biotechnology |
Zdroj: | Mastrochirico Filho, V A, Borges, C H S, Freitas, M V, Ariede, R B, Pilarski, F, Utsunomia, R, Carvalheiro, R, Gutierrez, A, Penaloza, C, Yanez, J M, Houston, R & Hashimoto, D T 2020, ' Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus) ', BMC Genomics . https://doi.org/10.1186/s12864-020-07090-z BMC Genomics, Vol 21, Iss 1, Pp 1-13 (2020) BMC Genomics Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
DOI: | 10.1186/s12864-020-07090-z |
Popis: | Made available in DSpace on 2021-06-25T10:35:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-09-29 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Background: Pacu (Piaractus mesopotamicus) is one of the most important Neotropical aquaculture species from South America. Disease outbreaks caused by Aeromonas hydrophila infection have been considered significant contributors to the declining levels of pacu production. The current implementation of genomic selection for disease resistance has been adopted as a powerful strategy for improvement in fish species. This study aimed to investigate the genetic architecture of resistance to A. hydrophila in pacu via Genome-Wide Association Study (GWAS), the identification of suggestive Quantitative Trait Loci (QTLs) and putative genes associated with this trait. The genetic data were obtained from 381 juvenile individuals belonging to 14 full-sibling families. An experimental challenge was performed to gain access to the levels of genetic variation for resistance against the bacteria using the following trait definitions: Binary test survival (TS) and time of death (TD). Results: The analyses of genetic parameters estimated moderate heritability (h 2 ) for both resistance traits: 0.20 (± 0.09) for TS and 0.35 (± 0.15) for TD. A linkage map for pacu was developed to enable the GWAS, resulting in 27 linkage groups (LGs) with 17,453 mapped Single Nucleotide Polymorphisms (SNPs). The length of the LGs varied from 79.95 (LG14) to 137.01 (LG1) cM, with a total map length of 2755.60 cM. GWAS identified 22 putative QTLs associated to A. hydrophila resistance. They were distributed into 17 LGs, and were considered suggestive genomic regions explaining > 1% of the additive genetic variance (AGV) for the trait. Several candidate genes related to immune response were located close to the suggestive QTLs, such as tbk1, trim16, Il12rb2 and lyz2. Conclusion: This study describes the development of the first medium density linkage map for pacu, which will be used as a framework to study relevant traits to the production of this species. In addition, the resistance to A. hydrophila was found to be moderately heritable but with a polygenic architecture suggesting that genomic selection, instead of marker assisted selection, might be useful for efficiently improving resistance to one of the most problematic diseases that affects the South American aquaculture. Saõ Paulo State University (Unesp) Aquaculture Center of Unesp, Via de Acesso Prof. Paulo Donato Castellane, s/n Universidade Federal Rural Do Rio de Janeiro Saõ Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences National Council for Scientific and Technological Development (CNPq) Roslin Institute and Royal (Dick) School of Veterinary Studies University of Edinburgh Facultad de Ciencias Veterinarias y Pecuarias Universidad de Chile Saõ Paulo State University (Unesp) Aquaculture Center of Unesp, Via de Acesso Prof. Paulo Donato Castellane, s/n Saõ Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences |
Databáze: | OpenAIRE |
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