κ-Carrageenan Oligosaccharides Inhibit the Inflammation of Lipopolysaccharide-Activated Microglia Via TLR4/NF-κB and p38/JNK MAPKs Pathways
| Autor: | Li-Ming Jin, Hai-Ge Wu, Bai-Xiang Wang, Ying Bai, Zi-Ang Yao, Cheng-Zhu Fu, Ling Xu |
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| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide viruses p38 mitogen-activated protein kinases Nitric Oxide Synthase Type II Oligosaccharides Inflammation Carrageenan Nitric Oxide Biochemistry p38 Mitogen-Activated Protein Kinases Proinflammatory cytokine Cellular and Molecular Neuroscience chemistry.chemical_compound medicine Humans Microglia Chemistry NF-kappa B Neurodegenerative Diseases General Medicine biochemical phenomena metabolism and nutrition Cell biology Toll-Like Receptor 4 medicine.anatomical_structure TLR4 Tumor necrosis factor alpha Signal transduction medicine.symptom |
| Zdroj: | Neurochemical research. 47(2) |
| ISSN: | 1573-6903 |
| Popis: | Microglial inflammation plays an essential role in neurodegenerative disease. Our previous studies had shown that κ-carrageenan oligosaccharides (KOS) could inhibit the excessive activation of microglia that induced by LPS, while the interrelated mechanisms were still indistinct. Therefore, we detected the inflammatory signaling pathway on LPS-activated microglia that pretreat by different content of KOS to reveal the mechanism on KOS's inhibition of microglia inflammatory response. ELISA was used to detect the effects of KOS on the secretion of interleukin-1 (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and prostaglandin E2 (PG-2) by LPS-activated microglia, respectively. The production of reactive oxygen species (ROS) and nitric oxide (NO) in microglia cells was detected by flow cytometry, and the protein expression of immunoinflammation-related signaling pathways were detected by Western Blot. The results showed that KOS could significantly protected the microglia from the over-activated inflammatory by inhibiting the release of inflammatory cytokines and the oxidative stress response. And KOS could reduce the expression of the protein that related to the TLR4/NF-κB and p38/JNK MAPKs pathways activated by LPS in microglia. However, there may be no specific target of KOS in cells. Therefore, KOS, a natural algal source oligosaccharide, has immunomodulatory effects and can be used as a potential intervention therapy for inflammatory related neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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