Effect of Vitamin C on pre-osteoblast gene expression
| Autor: | Furio Pezzetti, F. Illiano, Adriano Piattelli, Francesco Carinci, Gregorio Laino, Giordano Stabellini, E. Farina, Anna Spina, Alfredo De Rosa, Vittoria Perrotti, Annalisa Palmieri |
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| Přispěvatelé: | Carinci F., Pezzetti F., Spina A.M., Palmieri A., Laino G., De Rosa A., Farina E., Illiano F., Stabellini G., Perrotti V., Piattelli A., Carinci, F, Pezzetti, F, Spina, Annamaria, Palmieri, A, Laino, Gregorio, DE ROSA, Alfredo, Farina, E, Illiano, F, Stabellini, G, Perrotti, V, Piattelli, A. |
| Jazyk: | italština |
| Rok vydání: | 2005 |
| Předmět: |
Cell signaling
GENE EXPRESSION Morphogenesis Down-Regulation Biology Ascorbic acid DNA micro-array Gene expression Gene profiling Pre-osteoblast Stem cell NO Mice MICROARRAY medicine Animals Protein kinase A PRE-OSTEOBLAST General Dentistry Transcription factor Cell Proliferation Oligonucleotide Array Sequence Analysis Osteoblasts Cell growth Gene Expression Profiling Stem Cells Cell Differentiation Osteoblast 3T3 Cells Cell Biology General Medicine Molecular biology Up-Regulation VITAMIN C medicine.anatomical_structure Gene Expression Regulation Otorhinolaryngology ASCORBIC ACID |
| Popis: | Ascorbic acid (AA), also known as Vitamin C, is a cofactor required for the function of several hydroxylases. It is not synthesised in humans and has to be provided by diet. Its absence is responsible for scurvy, a condition related to the defective synthesis of collagen by the reduced function of prolylhydroxylase. AA is also a risk factor for periodontal disease. Recently, it has been shown that AA induces embryonic stem cells to differentiate into osteoblasts. The mechanism by which AA sustains pre-osteoblast proliferation and commitment is mediated through the synthesis of collagen type I, interaction with alpha2- and beta1-integrin, activation of the mitogen-activated protein kinase pathway, and phosphorylation of osteoblast-specific transcription factors. However, the multifunctional role of AA is not fully elucidated. MC3T3-E1 mouse calvaria-derived cell line is a well-defined in vitro model of pre-osteoblast differentiation, and AA is essential for the proliferation and differentiation of MC3T3-E1. By using DNA micro-arrays containing 15,000 genes, we identified several genes in MC3T3-E1 cultured with AA for 24h whose expression was significantly up or downregulated. The differentially expressed genes covered a broad range of functional activities: (1) cell growth; (2) metabolism; (3) morphogenesis; (4) cell death; (5) cell communication. The data reported are, to our knowledge, the first genetic portrait of early stage stimulation of pre-osteoblasts by AA, and may be relevant to better understand the molecular mechanism of pre-osteoblast proliferation and commitment. Elucidation of the molecular mechanism has important clinical implications because it may facilitate the correct use of AA to accelerate bone regeneration. |
| Databáze: | OpenAIRE |
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