Determination of oxygen affinity of respiratory systems using oxyhemoglobin as oxygen donor
| Autor: | M. Satre, O. Bârzu |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Oxygen donor
Kinetics Biophysics Analytical chemistry chemistry.chemical_element Mitochondria Liver Biochemistry Oxygen affinity Oxygen Hemoglobins Spectrophotometry Adrenal Glands medicine Animals Respiratory system Molecular Biology medicine.diagnostic_test Cell Biology Mitochondria chemistry Cattle Mitosporic Fungi Hemoglobin Saturation (chemistry) |
| Zdroj: | Analytical Biochemistry. 36:428-433 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(70)90379-9 |
| Popis: | A new method for measuring the K m for oxygen of respiratory systems using oxyhemoglobin as oxygen donor and indicator is described. The rate of HbO 2 reduction at very low oxygen pressure (fractional saturation of hemoglobin with oxygen being smaller than 1%) with a dual wavelength spectrophotometer (Δλ = 560-540 nm) was measured. The results obtained with three sources of mitochondria are in good agreement with those reported by other authors. |
| Databáze: | OpenAIRE |
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