Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysis
| Autor: | Cipriano Carrero-Carralero, Ana I. Ruiz-Matute, Andrea Martín-Ortiz, Rosa Lebrón-Aguilar, Oswaldo Hernández-Hernández, María Luz Sanz, Francisco J. Moreno |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Ministerio de Economía, Industria y Competitividad (España), Comunidad de Madrid, Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2019 |
| Předmět: |
Chemical structure
Diagnostic fragment ions 010402 general chemistry Mass spectrometry Disaccharides 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Liquid chromatography-multiple-stage mass spectrometry Monomeric units Tandem Mass Spectrometry chemistry.chemical_classification Chromatography Hydrophilic interaction chromatography 010401 analytical chemistry Organic Chemistry Glycosidic bond General Medicine Carbohydrate 0104 chemical sciences Molecular Weight Monomer chemistry Polymerization Mass spectrum Hydrophobic and Hydrophilic Interactions Trisaccharides Glycosidic linkage Chromatography Liquid |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1873-3778 2016-8047 |
| Popis: | Oligosaccharides are gaining importance because of their beneficial properties in human health. They normally appear in natural and synthetic products as complex mixtures of different monomeric units, glycosidic linkages and degrees of polymerization, being disaccharides and trisaccharides usually the most abundant ones. Although liquid chromatography-mass spectrometry is a useful technique for oligosaccharides analysis, the similarity of their structures makes difficult their characterization. Moreover, there is still scarce information about the relationship between carbohydrate chemical structure, mass spectra and chromatographic data. Then, in this work, chromatographic parameters for 23 disaccharides with different linkages and monomeric units (glucose, galactose, mannose and fructose) were determined using porous graphitized and hydrophilic interaction liquid chromatography columns. Moreover, diagnostic ions of these disaccharides obtained by tandem mass spectra (MS) were established by stepwise linear discriminant analysis. The relationship between carbohydrate chemical structure and their chromatographic retention data and characteristic ions obtained by multiple-stage mass spectrometry (MS) was successful in establishing some specific criteria that allowed the characterization of trisaccharides with different structural features. This work was supported by Ministerio de Economía, Industria y Competitividad (MINECO) of Spain (projects AGL2016-80475-R and AGL2017-84614-C2-1-R) and by the Comunidad of Madrid and European funding from FSE and FEDER programs (project S2018/BAA-4393, AVANSECAL-II-CM). |
| Databáze: | OpenAIRE |
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