Simultaneous effects of endocrine disruptor bisphenol A and flavonoid fisetin on progesterone production by granulosa cells

Autor: Sona Scsukova, Alzbeta Mlynarcikova
Rok vydání: 2018
Předmět:
0301 basic medicine
endocrine system
medicine.medical_specialty
Bisphenol A
Flavonols
Cell Survival
Swine
Health
Toxicology and Mutagenesis
Flavonoid
Steroid Isomerases
Endocrine Disruptors
Toxicology
Inhibitory postsynaptic potential
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Phenols
Multienzyme Complexes
Internal medicine
medicine
Animals
Cholesterol Side-Chain Cleavage Enzyme
Benzhydryl Compounds
Cells
Cultured
Progesterone
Flavonoids
Pharmacology
chemistry.chemical_classification
Granulosa Cells
030219 obstetrics & reproductive medicine
Caspase 3
Progesterone Reductase
urogenital system
Chemistry
Cholesterol side-chain cleavage enzyme
General Medicine
Phosphoproteins
Progesterone synthesis
030104 developmental biology
Endocrinology
Endocrine disruptor
Female
hormones
hormone substitutes
and hormone antagonists
Function (biology)
Fisetin
Zdroj: Environmental Toxicology and Pharmacology. 59:66-73
ISSN: 1382-6689
DOI: 10.1016/j.etap.2018.03.001
Popis: In the present study, we aimed to examine effects of different concentrations of the endocrine disruptor Bisphenol A (BPA; 1 nM, 1 μM, 100 μM) and the flavonoid fisetin (1, 10, 25, 50 μM), individually and in combinations, on steroidogenic function of porcine ovarian granulosa cells (GCs) represented by progesterone production. We confirmed that BPA inhibited progesterone production by GCs at the highest concentration. Fisetin reduced gonadotropin-stimulated progesterone synthesis dose-dependently, and in this manner, fisetin impaired progesterone production when added to BPA-treated GCs. The mechanisms of the inhibitory effects of the combinations included a significant down-regulation of the key steroidogenesis-related genes ( STAR , CYP11A1 , HSD3B ). Our findings suggest for the first time that fisetin might interfere with ovarian steroidogenesis, and might not have beneficial but rather aggravating effects in terms of modulating progesterone synthesis altered by high concentrations of BPA.
Databáze: OpenAIRE
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