Separation of constituents from Bergenia stracheyi (Hook. F.Thoms.) Engl. by high-speed countercurrent chromatography with elution mode and its antidiabetic and antioxidant in vitro evaluation
Autor: | Long Chen, Shahid Aziz, Daijie Wang, Yue Hou, Atiqa Sulaiman, Zhao Li, Iftikhar Ali, Hidayat Hussain, Xin Zheng, Li Cui |
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Rok vydání: | 2020 |
Předmět: |
Antioxidant
Cell Survival medicine.medical_treatment Filtration and Separation 010402 general chemistry 01 natural sciences Antioxidants Analytical Chemistry chemistry.chemical_compound Countercurrent chromatography medicine Humans Hypoglycemic Agents Gallic acid Countercurrent Distribution Chromatography High Pressure Liquid Chromatography Bergenia stracheyi biology Molecular Structure 010405 organic chemistry Chemistry Elution Saxifragaceae Bergenin Hep G2 Cells Hydrogen Peroxide biology.organism_classification 0104 chemical sciences Oxidative Stress Glucose Phytochemical Drugs Chinese Herbal |
Zdroj: | Journal of separation scienceREFERENCES. 44(3) |
ISSN: | 1615-9314 |
Popis: | Diabetes, a metabolic disorder, is caused by a high blood sugar level. Diabetes is an increasing health issue and search for potent antidiabetic agents is desirable. Owing to its ethnomedicinal value, the Himalayan perennial herb Bergenia stracheyi (Hook. f. & Thoms.) Engl. (Saxifragaceae Juss) is used to treat diabetes. Herein, an efficient high-speed countercurrent chromatography with elution mode is reported for separation of active compounds from B. stracheyi. In current investigation, six main compounds including β-arbutin (1), bergenin (2), 6-O-galloylarbutin (3), gallic acid (4), 11-O-galloylbergenin (5), and (-)-epicatechin 3-O-gallate (6) with above 95% purity were efficiently separated in a single run using biphasic tert-butyl methyl ether/n-butanol/methanol/water (1:3:1:5, v/v/v/v) solvent system. The structures of these compounds were characterized using spectral techniques and compared with the literature. Antidiabetic and antioxidant activities evaluation of the study samples showed that β-arbutin (1) and 6-O-galloylarbutin (3) have a significant protective effect, especially at high dose against hydrogen peroxide induced oxidative injury. Our results might help further in-depth phytochemical and biological evaluation studies in search of potent antidiabetic compounds from B. stracheyi. |
Databáze: | OpenAIRE |
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