| Autor: |
Linghui, Zou, Zhongbin, Zhang, Jinqing, Chen, Xu, Yang, Yuyang, Li, Jing, Tang, Xiaolu, Du, Ling, Tang, Dan, Liang, Xiaoyong, Zhu, Jianfang, Feng, Wenya, Ding |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
AAPS PharmSciTech. 23 |
| ISSN: |
1530-9932 |
| Popis: |
β-Cyclodextrin (CD) and chitosan (CS) have attracted great attention due to their unique properties and structures. β-Cyclodextrin-grafted chitosan (CD-CS) has been widely used as a drug carrier to prepare nano-formulations for drug delivery. However, few researches have been conducted to investigate the effect of CD-CS as an excipient on cellular uptake and intestinal absorption. Herein, Caco-2 cells were used to investigate the influence of CD-CS on cellular uptake. The MTT assay showed that CD-CS was non-toxic to Caco-2 cells in concentrations ranging from 15.62 to 125 μg/mL. Confocal laser microscopy and flow cytometry measurements indicated that the uptake ability of Caco-2 cells was significantly enhanced after being treated with CD-CS at a concentration of 31.25 μg/mL or incubation for 0.5 h, and the uptake enhancement gradually increased with increasing CD-CS concentration and incubation time. The Caco-2 monolayer cell model and the everted intestinal sac method were employed to preliminarily explore the mechanism of the improved intestinal absorption. The results demonstrated that CD-CS might open the tight junctions and enhance the clathrin-dependent endocytosis, macro-pinocytosis, and phagocytosis of the intestinal epithelial cells. Such findings can serve as references and inspiration for the design of absorption enhancers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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