Enhanced protein synthesis at the liver microsomal level in emetine-pretreated rats
| Autor: | D. Szapary, W.R. Jondorf |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Emetine
Phenylalanine Biophysics Cycloheximide Biology Biochemistry chemistry.chemical_compound Leucine In vivo Microsomes Protein biosynthesis medicine Animals Magnesium Amino Acids Molecular Biology chemistry.chemical_classification Carbon Isotopes Acetoxycycloheximide In vitro Rats Amino acid Liver chemistry Protein Biosynthesis Microsome Female medicine.drug |
| Zdroj: | Archives of Biochemistry and Biophysics. 126:892-904 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(68)90483-9 |
| Popis: | Emetine has different effects on l -(14C)-amino acid incorporation into protein at the liver microsomal level in the rat, depending on whether the experiments are performed in vitro or in vivo. Pretreatment with emetine in vivo, stimulates the in vitro amino acid incorporating activity of liver microsomal fractions subsequently prepared from treated rats. This stimulating effect which is most pronounced at 24 hr after injection of the drug at a dose level of 10 mg/kg is not dependent on sex or other hormonal factors, and is not additive with the increase of amino acid incorporating activity brought about by simultaneous pretreatment with cycloheximide or with acetoxycycloheximide. The stimulation, which is associated predominantly with the microsomal fraction appears to be related to an emetine-dependent stabilization of the activity of the in vitro incubation system overall, and of the endogenous mRNA-activity in particular. When emetine is added directly to the in vitro amino acid incorporating system containing liver microsomal preparations from control or emetine-pretreated rats, incorporation by both preparations can be inhibited completely. The same amount of emetine causing complete inhibition of labeled amino acid uptake when added to the system, does not however inhibit the release of labeled nascent polypeptide from liver microsomal preparations obtained from rats pre-labeled with l -(14C)-leucine in vivo, and then incubated in the in vitro system. The incorporation of labeled amino acid into rat liver protein in vivo is inhibited by pretreatment of rats with emetine. Many of these effects with emetine can be compared with those brought about in corresponding experiments with cycloheximide and other glutarimide antibiotics. |
| Databáze: | OpenAIRE |
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