Qualitative and Quantitative Characteristics of Rotavirus-Specific CD8 T Cells Vary Depending on the Route of Infection▿
| Autor: | Harry B. Greenberg, Janina Q. Jiang, Xiao-Song He, Ningguo Feng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Rotavirus
Adoptive cell transfer Immunology Spleen Biology CD8-Positive T-Lymphocytes medicine.disease_cause Microbiology Rotavirus Infections Feces Interferon-gamma Mice Peyer's Patches Receptors CCR Lysosomal-Associated Membrane Protein 1 T-Lymphocyte Subsets Virology Lysosomal-Associated Membrane Protein 2 medicine Cytotoxic T cell Animals Interferon gamma Lymphocyte function-associated antigen 1 Lung Receptors CXCR6 Homeodomain Proteins Mice Knockout Receptors CXCR Blood Cells T lymphocyte Flow Cytometry Adoptive Transfer Lymphocyte Function-Associated Antigen-1 Virus Shedding Mice Inbred C57BL medicine.anatomical_structure Blood Insect Science Pathogenesis and Immunity CD8 medicine.drug |
| Popis: | CD8 T-cell response provides an important defense against rotavirus, which infects a variety of systemic locations in addition to the gut. Here we investigated the distribution, phenotype, and function of rotavirus-specific CD8 T cells in multiple organs after rotavirus infection initiated via the intranasal, oral, or intramuscular route. The highest level of virus-specific CD8 T cells was observed in the Peyer's patches of orally infected mice and in the lungs of intranasally infected animals. Very low levels of virus-specific CD8 T cells were detected in peripheral blood or spleen irrespective of the route of infection. Rotavirus-specific CD8 T cells from Peyer's patches of orally infected mice expressed high levels of CCR9, while CXCR6 and LFA-1 expression was associated with virus-specific CD8 T cells in lungs of intranasally infected mice. Oral infection induced the highest proportion of gamma interferon − CD107a/b + CD8 T cells in Peyer's patches. When equal numbers of rotavirus-specific CD8 T cells were transferred into Rag-1 knockout mice chronically infected with rotavirus, the donor cells derived from Peyer's patches of orally infected mice were more efficient than those derived from lungs of intranasally infected animals in clearing intestinal infection. These results suggest that different routes of infection induce virus-specific CD8 T cells with distinct homing phenotypes and effector functions as well as variable abilities to clear infection. |
| Databáze: | OpenAIRE |
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