[Analysis of tetracyclines in honey and royal jelly by LC/MS/MS]
| Autor: | Tamio Maitani, Masakazu Horie, Mitsunori Murayama, Rie Ishii |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Chlortetracycline
food.ingredient Chromatography Chemistry Fatty Acids General Medicine Oxytetracycline Honey Tandem mass spectrometry Sensitivity and Specificity Drug Residues Anti-Bacterial Agents food Sep-Pak C18 Tandem Mass Spectrometry Tetracyclines Royal jelly medicine Food Analysis medicine.drug Chromatography Liquid |
| Zdroj: | Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan. 47(6) |
| ISSN: | 0015-6426 |
| Popis: | A simple and accurate method using liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) was developed for the determination of tetracyclines (TCs), i.e., oxytetracycline (OTC), chlortetracycline (CTC) and tetracycline (TC), in honey and royal jelly. Mass spectral acquisition was performed in the positive mode. In LC separation, L-column ODS and 0.01% formic acid-acetonitrile were used as the column and mobile phase, respectively. TCs in a honey sample were diluted with water, while TCs in royal jelly were extracted with 2% metaphosphoric acid-methanol (6:4). They were cleaned up with Oasis HLB and Sep Pak C18 cartridges, respectively. The quantification limits of TC, OTC, and CTC were 5, 5, and 10 ng/g, respectively, while those in royal jelly were 25, 25, and 50 ng/g, respectively. The recoveries of TCs from both honey and royal jelly were 75-120%. |
| Databáze: | OpenAIRE |
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