Interleukin-1 Facilitates Airway Epithelial Migration in Response to Injury
| Autor: | Joseph A. Gardella, Debra A. Tristram, Lynn Sigurdson, Wesley L. Hicks, Robert Hard, Leon Hall, Frank V. Bright |
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| Rok vydání: | 2003 |
| Předmět: |
Respiratory Mucosa
Cell division Swine medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Epithelial cell migration Antibodies Andrology Organ Culture Techniques Cell Movement medicine Animals Cells Cultured Dose-Response Relationship Drug biology Interleukin Epithelium Trachea medicine.anatomical_structure Cytokine Otorhinolaryngology Cell culture Immunology biology.protein Antibody Cell Division Interleukin-1 |
| Zdroj: | The Laryngoscope. 113:243-247 |
| ISSN: | 0023-852X |
| Popis: | Objective To test the hypothesis that interleukin (IL)-1 plays a permissive role in respiratory epithelial cell migration and proliferation. Study Design Primary cultures of porcine respiratory epithelial cells or tracheal organ explants were cultured in the presence or absence of function-blocking antibodies to IL-1. Areas of epithelial cell outgrowth were determined in control and antibody-treated organ explants daily for 4 days. At intervals, cultured cells were collected for cell counting and viability determination. Time course and dose–response curves were constructed for control and antibody-treated groups. Results Interleukin-1 secretion into culture supernatants increased sharply from days 3 to 7. Outgrowths from tracheal explants were reduced by greater than 60% by single antibody treatment, and by over 90% by treatment with antibodies to both IL-1α and -1β by day 4 of culture. Function-blocking antibodies to IL-1 significantly reduced cell number by day 7 of culture. Conclusions Interleukin-1 is produced by respiratory epithelial cells in culture during log phase growth and plays a permissive role in cell migration and proliferation. |
| Databáze: | OpenAIRE |
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