Ecotoxicity assessment of microcystins from freshwater samples using a bioluminescent cyanobacterial bioassay
| Autor: | Samuel Cirés, Andreu Rico, Antonio Quesada, Gerardo Pulido-Reyes, Ramsy Agha, Keila Martín-Betancor, Miguel González-Pleiter, Francisca Fernández-Piñas, Lars Wörmer, Francisco Leganés |
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| Přispěvatelé: | UAM. Departamento de Biología |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cyanobacteria
Environmental Engineering Microcystins Health Toxicology and Mutagenesis Microorganism 0208 environmental biotechnology Fresh Water Context (language use) Additive Index 02 engineering and technology 010501 environmental sciences Ecotoxicology 01 natural sciences Microbiology Environmental Chemistry Bioassay Bioluminescence 0105 earth and related environmental sciences biology Chemistry Anabaena Public Health Environmental and Occupational Health General Medicine General Chemistry Intracellular Free Ca2+ Cyanotoxin QS Ecology biology.organism_classification Biología y Biomedicina / Biología QD Chemistry Pollution humanities 020801 environmental engineering Biological Assay Calcium Ecotoxicity |
| Zdroj: | Repositorio Institucional del Instituto IMDEA Agua instname Instituto IMDEA Agua (IMDEA AGUA) |
| DOI: | 10.1016/j.chemosphere.2019.124966 |
| Popis: | The hepatotoxic cyanotoxins microcystins (MCs) are emerging contaminants naturally produced by cyanobacteria. Yet their ecological role remains unsolved, previous research suggests that MCs have allelopathic effects on competing photosynthetic microorganisms, even eliciting toxic effects on other freshwater cyanobacteria. In this context, the bioluminescent recombinant cyanobacterium Anabaena sp. PCC7120 CPB4337 (hereinafter Anabaena) was exposed to extracts of MCs. These were obtained from eight natural samples from freshwater reservoirs that contained MCs with a concentration range of 0.04–11.9 μg MCs L−1. MCs extracts included the three most common MCs variants (MC-LR, MC-RR, MC-YR) in different proportions (MC-LR: 100–0%; MC-RR: 100–0%; MC-YR: 14.2–0%). The Anabaena bioassay based on bioluminescence inhibition has been successfully used to test the toxicity of many emerging contaminants (e.g., pharmaceuticals) but never for cyanotoxins prior to this study. Exposure of Anabaena to MCs extracts induced a decrease in its bioluminescence with effective concentration decreasing bioluminescence by 50% ranging from 0.4 to 50.5 μg MC L−1 in the different samples. Bioluminescence responses suggested an interaction between MCs variants which was analyzed via the Additive Index method (AI), indicating an antagonistic effect (AI < 0) of MC-LR and MC-RR present in the samples. Additionally, MC extracts exposure triggered an increase of intracellular free Ca2+ in Anabaena. In short, this study supports the use of the Anabaena bioassay as a sensitive tool to assess the presence of MCs at environmentally relevant concentrations and opens interesting avenues regarding the interactions between MCs variants and the possible implication of Ca2+ in the mode of action of MCs towards cyanobacteria |
| Databáze: | OpenAIRE |
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