Knockout of Auxin Response Factor SlARF4 Improves Tomato Resistance to Water Deficit

Autor: Mohammed Zouine, Xiaoyang Zhu, Mondher Bouzayen, Canye Yu, Guojian Hu, Yanwei Hao, Xiaojuan Liu, Caiyu Wu, Riyuan Chen, Lin Chen, Mengyi Chen
Přispěvatelé: South China Agricultural University (SCAU), Chinese Academy of Science (CAS), Génomique et Biotechnologie des Fruits (GBF), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), National Natural Science Foundation of China (31870286) and (31902013), Natural Science Foundation of Guangdong Province (2017A030313114) and (2018A030310205), General Project of Guangzhou city (201804010031), International Science and Technology Cooperation Major Project Cultivation Special Fund of SCAU (2019SCAUGH05)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Chlorophyll
0106 biological sciences
0301 basic medicine
Mutant
drought
tomato
01 natural sciences
lcsh:Chemistry
chemistry.chemical_compound
Solanum lycopersicum
Gene Expression Regulation
Plant
Malondialdehyde
Guard cell
heterocyclic compounds
RNA-Seq
Abscisic acid
lcsh:QH301-705.5
Spectroscopy
Glucuronidase
Plant Proteins
water deficit
2. Zero hunger
chemistry.chemical_classification
biology
food and beverages
General Medicine
Plants
Genetically Modified
6. Clean water
Droughts
Computer Science Applications
Cell biology
ABA
Catalase
Signal Transduction
Real-Time Polymerase Chain Reaction
Article
Catalysis
Inorganic Chemistry
Superoxide dismutase
03 medical and health sciences
Auxin
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Physical and Theoretical Chemistry
Molecular Biology
Indoleacetic Acids
Arabidopsis Proteins
Gene Expression Profiling
Organic Chemistry
fungi
Wild type
Water
SlARF4
Vascular bundle
Plant Leaves
030104 developmental biology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Mutation
Plant Stomata
biology.protein
CRISPR-Cas Systems
Transcriptome
auxin
Abscisic Acid
Transcription Factors
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 3347, p 3347 (2021)
International Journal of Molecular Sciences
International Journal of Molecular Sciences, MDPI, 2021, 22 (7), pp.3347. ⟨10.3390/ijms22073347⟩
Volume 22
Issue 7
ISSN: 1661-6596
1422-0067
Popis: Auxin response factors (ARFs) play important roles in various plant physiological processes
however, knowledge of the exact role of ARFs in plant responses to water deficit is limited. In this study, SlARF4, a member of the ARF family, was functionally characterized under water deficit. Real-time fluorescence quantitative polymerase chain reaction (PCR) and β-glucuronidase (GUS) staining showed that water deficit and abscisic acid (ABA) treatment reduced the expression of SlARF4. SlARF4 was expressed in the vascular bundles and guard cells of tomato stomata. Loss of function of SlARF4 (arf4) by using Clustered Regularly Interspaced Short Palindromic Repeats/Cas 9 (CRISPR/Cas 9) technology enhanced plant resistance to water stress and rehydration ability. The arf4 mutant plants exhibited curly leaves and a thick stem. Malondialdehyde content was significantly lower in arf4 mutants than in wildtype plants under water stress
furthermore, arf4 mutants showed higher content of antioxidant substances, superoxide dismutase, actual photochemical efficiency of photosystem II (PSII), and catalase activities. Stomatal and vascular bundle morphology was changed in arf4 mutants. We identified 628 differentially expressed genes specifically expressed under water deficit in arf4 mutants
six of these genes, including ABA signaling pathway-related genes, were differentially expressed between the wildtype and arf4 mutants under water deficit and unlimited water supply. Auxin responsive element (AuxRE) elements were found in these genes’ promoters indicating that SlARF4 participates in ABA signaling pathways by regulating the expression of SlABI5/ABF and SCL3, thereby influencing stomatal morphology and vascular bundle development and ultimately improving plant resistance to water deficit.
Databáze: OpenAIRE
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