Knockout of Auxin Response Factor SlARF4 Improves Tomato Resistance to Water Deficit
Autor: | Mohammed Zouine, Xiaoyang Zhu, Mondher Bouzayen, Canye Yu, Guojian Hu, Yanwei Hao, Xiaojuan Liu, Caiyu Wu, Riyuan Chen, Lin Chen, Mengyi Chen |
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Přispěvatelé: | South China Agricultural University (SCAU), Chinese Academy of Science (CAS), Génomique et Biotechnologie des Fruits (GBF), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), National Natural Science Foundation of China (31870286) and (31902013), Natural Science Foundation of Guangdong Province (2017A030313114) and (2018A030310205), General Project of Guangzhou city (201804010031), International Science and Technology Cooperation Major Project Cultivation Special Fund of SCAU (2019SCAUGH05) |
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Chlorophyll
0106 biological sciences 0301 basic medicine Mutant drought tomato 01 natural sciences lcsh:Chemistry chemistry.chemical_compound Solanum lycopersicum Gene Expression Regulation Plant Malondialdehyde Guard cell heterocyclic compounds RNA-Seq Abscisic acid lcsh:QH301-705.5 Spectroscopy Glucuronidase Plant Proteins water deficit 2. Zero hunger chemistry.chemical_classification biology food and beverages General Medicine Plants Genetically Modified 6. Clean water Droughts Computer Science Applications Cell biology ABA Catalase Signal Transduction Real-Time Polymerase Chain Reaction Article Catalysis Inorganic Chemistry Superoxide dismutase 03 medical and health sciences Auxin [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Physical and Theoretical Chemistry Molecular Biology Indoleacetic Acids Arabidopsis Proteins Gene Expression Profiling Organic Chemistry fungi Wild type Water SlARF4 Vascular bundle Plant Leaves 030104 developmental biology chemistry lcsh:Biology (General) lcsh:QD1-999 Mutation Plant Stomata biology.protein CRISPR-Cas Systems Transcriptome auxin Abscisic Acid Transcription Factors 010606 plant biology & botany |
Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 3347, p 3347 (2021) International Journal of Molecular Sciences International Journal of Molecular Sciences, MDPI, 2021, 22 (7), pp.3347. ⟨10.3390/ijms22073347⟩ Volume 22 Issue 7 |
ISSN: | 1661-6596 1422-0067 |
Popis: | Auxin response factors (ARFs) play important roles in various plant physiological processes however, knowledge of the exact role of ARFs in plant responses to water deficit is limited. In this study, SlARF4, a member of the ARF family, was functionally characterized under water deficit. Real-time fluorescence quantitative polymerase chain reaction (PCR) and β-glucuronidase (GUS) staining showed that water deficit and abscisic acid (ABA) treatment reduced the expression of SlARF4. SlARF4 was expressed in the vascular bundles and guard cells of tomato stomata. Loss of function of SlARF4 (arf4) by using Clustered Regularly Interspaced Short Palindromic Repeats/Cas 9 (CRISPR/Cas 9) technology enhanced plant resistance to water stress and rehydration ability. The arf4 mutant plants exhibited curly leaves and a thick stem. Malondialdehyde content was significantly lower in arf4 mutants than in wildtype plants under water stress furthermore, arf4 mutants showed higher content of antioxidant substances, superoxide dismutase, actual photochemical efficiency of photosystem II (PSII), and catalase activities. Stomatal and vascular bundle morphology was changed in arf4 mutants. We identified 628 differentially expressed genes specifically expressed under water deficit in arf4 mutants six of these genes, including ABA signaling pathway-related genes, were differentially expressed between the wildtype and arf4 mutants under water deficit and unlimited water supply. Auxin responsive element (AuxRE) elements were found in these genes’ promoters indicating that SlARF4 participates in ABA signaling pathways by regulating the expression of SlABI5/ABF and SCL3, thereby influencing stomatal morphology and vascular bundle development and ultimately improving plant resistance to water deficit. |
Databáze: | OpenAIRE |
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