Antegrade Versus Retrograde Isolated Lung Perfusion: Doxorubicin Uptake and Distribution in a Sarcoma Model
| Autor: | Hans-Beat Ris, Hua Yan, Thorsten Krueger, Andrea Kuemmerle, Walter Klepetko, Roger Stupp, Jean Pierre Ballini, Snezana Andrejevic-Blant, Laurent A. Decosterd, Youmin Pan |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Lung Neoplasms Isolated lung perfusion Cell Line Tumor medicine.artery Parenchyma medicine Animals Doxorubicin Antibiotics Antineoplastic Lung business.industry Respiratory disease Sarcoma respiratory system medicine.disease Xenograft Model Antitumor Assays Rats Inbred F344 Rats respiratory tract diseases medicine.anatomical_structure Chemotherapy Cancer Regional Perfusion Surgery Cardiology and Cardiovascular Medicine Bronchial artery business Perfusion medicine.drug |
| Zdroj: | The Annals of Thoracic Surgery. 82:2024-2030 |
| ISSN: | 0003-4975 |
| Popis: | Background Antegrade and retrograde doxorubicin-based isolated lung perfusions were compared in rodents bearing a sarcomatous tumor in the perfused lung. Inasmuch as these tumors derive their vascularization from the bronchial artery system, we hypothesized that retrograde isolated lung perfusion through the pulmonary vein might result in an improved tumor drug uptake. Methods Single-pass antegrade (n = 9) and retrograde (n = 9) isolated left lung perfusions were performed with 100 μg of doxorubicin in Fischer rats 10 days after subpleural tumor cell injection. The perfusion, washout, and recirculation times were 20, 10, and 60 minutes, respectively, followed by harvesting of the lung. The doxorubicin concentration and compartmental distribution in the tumor and in normal parenchyma of each perfused lung were measured by high-pressure liquid chromatography (6 animals of each group) and fluorescence microscopy (3 animals of each group). Results Doxorubicin concentration and pattern of doxorubicin-based fluorescence signaling were comparable for both perfusion techniques in normal lung tissue. Antegrade and retrograde isolated lung perfusion resulted in similar tumor drug uptake, which was lower than in normal lung parenchyma, and in weak and sporadic fluorescence signaling emerging from the tumor periphery and from blood vessels situated within the tumor tissue. Conclusions Retrograde isolated lung perfusion did not confer a better doxorubicin uptake in the tumor as compared with antegrade lung perfusion despite the fact that the tumor vascularization in this model is based on the bronchial artery circulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|