Differing effects of enalapril and losartan on renal medullary blood flow and renal interstitial hydrostatic pressure in spontaneously hypertensive rats
Autor: | Stephen A. W. Dukacz, Robert L. Kline |
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Rok vydání: | 1999 |
Předmět: |
Male
medicine.medical_specialty Mean arterial pressure Physiology Adrenergic beta-Antagonists Hydrostatic pressure Angiotensin-Converting Enzyme Inhibitors Blood Pressure Bradykinin Receptor Angiotensin Type 2 Losartan Receptor Angiotensin Type 1 Renal Circulation Angiotensin Receptor Antagonists Spontaneously hypertensive rat Enalapril Rats Inbred SHR Internal medicine Hydrostatic Pressure Laser-Doppler Flowmetry Internal Medicine medicine Animals cardiovascular diseases Antihypertensive Agents Kidney Medulla biology business.industry Angiotensin II Angiotensin-converting enzyme Rats Endocrinology ACE inhibitor cardiovascular system biology.protein Angiotensin I Cardiology and Cardiovascular Medicine business hormones hormone substitutes and hormone antagonists circulatory and respiratory physiology medicine.drug |
Zdroj: | Journal of Hypertension. 17:1345-1352 |
ISSN: | 0263-6352 |
Popis: | OBJECTIVE To determine the effect of short-term angiotensin converting enzyme inhibition (enalapril) or angiotensin II AT1 receptor blockade (losartan) on medullary hemodynamics in the spontaneously hypertensive rat (SHR). DESIGN Laser-Doppler flowmetry allowed for the characterization of medullary blood flow (MBF) over a wide range of renal arterial pressure (RAP), and was used for comparison among treatment groups. Renal interstitial hydrostatic pressure (RIHP) was also determined over a wide range of RAP. METHOD Enalapril or losartan was given to male 12-13-week-old SHR for 3 days (25 mg/kg per day in drinking water). Rats were anesthetized with Inactin, renal function was measured at resting levels of RAP and then RAP was varied over a range of 50-150 mmHg in 25 mmHg steps. MBF and RIHP were determined at each pressure. RESULTS Resting mean arterial pressure (MAP) (mmHg +/- SE) for enalapril- and for losartan-treated SHR [114 +/- 3 (n = 18) and 124 +/- 3 (n = 20), respectively] were both significantly lower than for untreated SHR [159 +/- 5 (n = 20)]. Renal function at resting levels of MAP was not significantly different among groups. Enalapril and losartan both increased MBF by 30% at levels of RAP of 125 mmHg and over. Enalapril did not alter the relation between RAP and RIHP, but losartan shifted the RAP versus RIHP curve by approximately 40 mmHg to lower levels of RAP. Acute administration of the B2 kinin receptor antagonist HOE 140 [20 microg/kg intravenous (i.v.) bolus, then 10 microg/kg per h i.v.] did not significantly alter MAP in any group. HOE 140 did not significantly alter MBF or RIHP in the untreated or losartan-treated SHR. MBF in enalapril-treated rats receiving HOE 140 was not significantly different from that of the enalapril-only group; however, the relation between RAP and RIHP was shifted to lower levels of RAP by approximately 45 mmHg. CONCLUSIONS Both enalapril and losartan increase MBF in SHR, suggesting that the medullary circulation of SHR is influenced by endogenous levels of angiotensin II. The failure of enalapril to increase RIHP in parallel with MBF appears to be due to an enhanced effect of kinins. |
Databáze: | OpenAIRE |
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