A novel in vitro method for the detection and characterization of photosensitizers
| Autor: | Silke Gerlach, Robert Dr. Schmucker, Klaus-Peter Wittern, Andreas Schepky, Yeliz Tepe, Nadine Karschuk, Hendrik Reuter, Wolfgang Pape, Horst Wenck |
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| Rok vydání: | 2010 |
| Předmět: |
Ultraviolet Rays
Immune Cells Immunology lcsh:Medicine Antigen-Presenting Cells Dermatitis Dermatology In Vitro Techniques Toxicology Models Biological Monocytes Flow cytometry Contact Dermatitis Immune system Immunotoxicology Molecular Cell Biology medicine Hypersensitivity Humans Cytotoxicity lcsh:Science Biology Cells Cultured CD86 Regulation of gene expression Multidisciplinary Photosensitizing Agents medicine.diagnostic_test Chemistry Allergy and Hypersensitivity lcsh:R Dose-Response Relationship Radiation Photosensitizing Agent Dendritic Cells Allergens Flow Cytometry In vitro Gene Expression Regulation Biophysics Leukocytes Mononuclear Medicine lcsh:Q B7-2 Antigen Phototoxicity Cytometry Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 5, Iss 12, p e15221 (2010) |
| ISSN: | 1932-6203 |
| Popis: | Photoactivation and binding of photoactive chemicals to proteins is a known prerequisite for the formation of immunogenic photoantigens and the induction of photoallergy. The intensive use of products and the availability of new chemicals, along with an increasing exposure to sun light contribute to the risk of photosensitizing adverse reactions. Dendritic cells (DC) play a pivotal role in the induction of allergic contact dermatitis. Human peripheral blood monocyte derived dendritic cells (PBMDC) were thus perceived as an obvious choice for the development of a novel in vitro photosensitization assay using the modulation of cell surface protein expression in response to photosensitizing agents. In this new protocol, known chemicals with photosensitizing, allergenic or non-allergenic potential were pre-incubated with PBMDCs prior to UVA irradiation (1 J/cm(2)). Following a 48 h incubation, the expression of the cell surface molecules CD86, HLA-DR and CD83 was measured by flow cytometry. All tested photosensitizers induced a significant and dose-dependent increase of CD86 expression after irradiation compared to non-irradiated controls. Moreover, the phototoxicity of the chemicals could also be determined. In contrast, (i) CD86 expression was not affected by the chosen irradiation conditions, (ii) increased CD86 expression induced by allergens was independent of irradiation and (iii) no PBMDC activation was observed with the non-allergenic control. The assay proposed here for the evaluation of the photoallergenic potential of chemicals includes the assessment of their allergenic, phototoxic and toxic potential in a single and robust test system and is filling a gap in the in vitro photoallergenicity test battery. |
| Databáze: | OpenAIRE |
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